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pcDNA4/TO-ORF24 3L_A-2xStrep

Plasmid

#138440

Purpose

Expresses full-length ORF24 3L_A with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

Strep

Expression

Mammalian

Mutation

Amino acids L73-L75 mutated to alanine

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 202-752-3xFLAG

Plasmid

#138424

Purpose

Expresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 202-752 (ORF24 KSHV (HHV-8))

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 202-752

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 3L_A-3xFLAG

Plasmid

#138425

Purpose

Expresses full-length ORF24 3L_A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids L73-L75 mutated to alanine

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L73A-3xFLAG

Plasmid

#138426

Purpose

Expresses full-length ORF24 L73A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid L73 mutated to alanine

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L74A-3xFLAG

Plasmid

#138427

Purpose

Expresses full-length ORF24 L74A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid L74 mutated to alanine

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L75A-3xFLAG

Plasmid

#138428

Purpose

Expresses full-length ORF24 L75A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid L75 mutated to alanine

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSHDY*in_PpetE:BCD2:AgBIS:Flag

Plasmid

#133971

Purpose

Heterologous, copper-inducible expression of (E)-α-bisabolene synthase from Abies grandis (AgBIS) in Synechocystis sp. PCC 6803. The AgBIS CDS is fused to a C-termial Flag-tag.

Depositor

Pia Lindberg

Article

Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

Insert

AgBIS:Flag

Use

Synthetic Biology

Tags

Flag

Expression

Bacterial

Mutation

inserted gene is codon optimized for Synechocyst…

Promoter

PpetE:BCD2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSHDY*in_PpetE:BCD2:MrBBS:Strep

Plasmid

#133973

Purpose

Heterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag.

Depositor

Pia Lindberg

Article

Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

Insert

MrBBS:StrepII

Use

Synthetic Biology

Tags

StrepII

Expression

Bacterial

Mutation

inserted gene is codon optimized for Synechocyst…

Promoter

PpetE:BCD2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6AP III (C41A, C46A, C840A)

Plasmid

#119318

Purpose

lentiviral vector for expression of HA-Tagged E6-AP III (C41A, C46A, C840A) in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP III (UBE3A Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

C41A, C46A, C840A

Promoter

CMV

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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His Puc (aa 1-393)

Plasmid

#52295

Purpose

Bacterial expression of His tagged Drosophila Puc fragment

Depositor

Brenda Schulman

Article

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Insert

Puc (puc Fly)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only
pDD2034

Plasmid

#101377

Purpose

Name: pBluescript-Δ611-843REV. Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), 611-843 REV.

Depositor

Dirk Dittmer

Article

Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

Insert

Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), 611-843 REV

Use

Unspecified

Tags

Expression

Mutation

Blue Heron Biotechnology Inc. bidirectional mutan…

Promoter

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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p6330 pHAGE-P-CMVt-N-HA-GAW-HERC2-F6

Plasmid

#100140

Purpose

Encodes amino acids 4421-4834 of HERC2.

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HERC2 (HERC2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

amino acids 4421-4834

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p6329 pHAGE-P-CMVt-N-HA-GAW-HERC2-F5

Plasmid

#100139

Purpose

Encodes amino acids 3550-4500 of HERC2.

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HERC2 (HERC2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

amino acids 3550-4500

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p6328 pHAGE-P-CMVt-N-HA-GAW-HERC2-F4

Plasmid

#100138

Purpose

Encodes amino acids 2600-3600 of HERC2.

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HERC2 (HERC2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

amino acids 2600-3600

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p6326 pHAGE-P-CMVt-N-HA-GAW-HERC2-F2

Plasmid

#100136

Purpose

Encodes amino acids 950-1750 of HERC2.

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HERC2 (HERC2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

amino acids 950-1750

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p6325 pHAGE-P-CMVt-N-HA-GAW-HERC2-F1

Plasmid

#100135

Purpose

Encodes amino acids 1-1000 of HERC2.

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HERC2 (HERC2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

amino acids 1-1000

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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AAT_g1 gRNA

Plasmid

#86005

Purpose

plasmid vector encoding for U6-driven AAT_g1 gRNA

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g1 sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pU6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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AAT_g2 gRNA

Plasmid

#86006

Purpose

plasmid vector encoding for U6-driven AAT_g2 gRNA (Z-allele specific)

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g2 sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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FCK-ChrimsonR-GFP

Plasmid

#59049

Purpose

Lentiviral expression of ChrimsonR under the CaMKII promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

K176R

Promoter

CaMKII

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pLX313-Firefly luciferase

Plasmid

#118017

Purpose

Constitutive expression of Firefly luciferase

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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