Skip to main content
Addgene
Showing: 1401 - 1420 of 47536 results

  1. pSBtet-RB-PE2

    Plasmid
    #173903
    Purpose
    SB-transposon with inducible expression of SpCas9 PE2
    Depositor
    Ronald Cohn
    Insert
    PE2
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173903-plasmid-map-sequence-id-346343

    Enlarge

  2. pKM510

    Plasmid
    #134227
    Purpose
    Retroviral plasmid expressing mCherry - HJURP
    Depositor
    Iain Cheeseman
    Insert
    mCherry-HJURP-LacI 1-304 (HJURP Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    134227-plasmid-map-sequence-id-271958

    Enlarge

  3. p-dCas9-VP64-Hygro

    Plasmid
    #104405
    Purpose
    transient expression of dCas9-VP64 fusion protein
    Depositor
    Stephan Beck
    Insert
    VP64
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104405-plasmid-map-sequence-id-204462

    Enlarge

  4. pCAGGS-mTagBFP2-T2A-iCreERT2 (PA)

    Plasmid
    #128174
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    128174-plasmid-map-sequence-id-247966

    Enlarge

  5. pDEST26-his-hSFRP1

    Plasmid
    #189539
    Purpose
    Protein expression of SFRP1 in mammalian cells.
    Depositor
    Haian Fu
    Insert
    secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    his
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    189539-plasmid-map-sequence-id-374149

    Enlarge

  6. pCAGGS-mTagBFP2-T2A-iCreERT2 (PV)

    Plasmid
    #128171
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PV N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    128171-plasmid-map-sequence-id-247962

    Enlarge

  7. pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH

    Plasmid
    #100050
    Purpose
    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV9
    Insert
    hChR2(E123T/H134R)
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    humanized, E123T/H134R
    Promoter
    CaMKIIa
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100050-plasmid-map-sequence-id-194451

    Enlarge

  8. pZBTB5-donor

    Plasmid
    #112303
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB5 with GFP
    Depositor
    Kevin White
    Insert
    ZBTB5 homology arms flanking EGFP-IRES-Neo cassette (ZBTB5 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112303-plasmid-map-sequence-id-228415

    Enlarge

  9. pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH

    Plasmid
    #100049
    Purpose
    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by hSynap promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV9
    Insert
    hChR2(E123T/H134R)
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    humanized, E123T/H134R
    Promoter
    hSynap
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100049-plasmid-map-sequence-id-194448

    Enlarge

  10. pHAGE-CMV-Rheb(D60I)-IRES-eGFP-W

    Plasmid
    #32521
    Depositor
    Mustafa Sahin
    Insert
    Dominant negative Rheb (D60I) (Rheb Rat)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D60I to make dominant negative
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  11. pAAV-CamKIIa-C1V1::FusionRed::Kv2.1

    Plasmid
    #102771
    Purpose
    "Activate neurons with 2p stimulation"
    Depositor
    Karel Svoboda
    Insert
    C1V1::FusionRed::Kv2.1
    Use
    AAV
    Tags
    FusionRed and Kv2.1 traficking domain
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    102771-plasmid-map-sequence-id-239474

    Enlarge

  12. pBS ZfHoxb1a

    Plasmid
    #27519
    Depositor
    Victoria Prince
    Insert
    Hoxb1a (hoxb1a Zebrafish)
    Use
    Riboprobe synthesis
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  13. SPIN3A

    Plasmid
    #74656
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SPIN3A (SPIN3 Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)

    Plasmid
    #137859
    Purpose
    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    137859-plasmid-map-sequence-id-274442

    Enlarge

  15. pZFX-donor

    Plasmid
    #112366
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZFX with GFP
    Depositor
    Kevin White
    Insert
    ZFX homology arms flanking EGFP-IRES-Neo cassette (ZFX Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112366-plasmid-map-sequence-id-229714

    Enlarge

  16. pBS ZfHoxa2b

    Plasmid
    #27514
    Depositor
    Victoria Prince
    Insert
    Hoxa2b (hoxa2b Zebrafish)
    Use
    Riboprobe synthesis
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  17. psicheck_NDUFA6_3UTR

    Plasmid
    #65886
    Purpose
    Luciferase assay vector, assessing regulation of NDUFA6 3' UTR
    Depositor
    Thomas Tuschl
    Insert
    NDUFA6 (NDUFA6 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pBS ZfHoxc6a

    Plasmid
    #27541
    Depositor
    Victoria Prince
    Insert
    Hoxc6a (hoxc6a Zebrafish)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 24, 2011
    Availability
    Academic Institutions and Nonprofits only

  19. INT_Cargo_T7_gutB

    Plasmid
    #212139
    Purpose
    Cargo plasmid for integrating PT7 expression cassette of xylitol dehydrogenase in in genome.Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    xylitol dehydrogenase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    212139-plasmid-map-sequence-id-424777

    Enlarge

  20. pCT03

    Plasmid
    #212150
    Purpose
    Plasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C.
    Depositor
    Kang Zhou
    Insert
    alanine dehydrogenase
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212150-plasmid-map-sequence-id-420600

    Enlarge

Showing: 1401 - 1420 of 47536 results