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Plasmid#138966PurposePeft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegansDepositorInsertwrmScarlet1-10
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#83946PurposeCas9-carrying vector with kanMX markerDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCoofy48
Plasmid#55186PurposeBacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTags6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…ExpressionBacterialMutationPromoterT7Available sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCoofy49
Plasmid#55187PurposeBacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and MBP tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTags10x His-Stop-OneStrep-Stop-S-tag-Stop-CBP-Stop-HP…ExpressionBacterialMutationPromoterT7Available sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NBid-PhoCl2c-CBid
Plasmid#164051PurposeOptogenetic manipulation of cell apoptosis using PhoCl2cDepositorInsertNBid-PhoCl2c-CBid
UseTagskozak sequenceExpressionMammalianMutationPromoterCMV promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-RhoBAST-MS2
Plasmid#196342PurposeOverexpression of circular RhoBAST-MS2DepositorInsertTornado-RhoBAST-MS2
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-C3-HA-Ubiquitin
Plasmid#28236DepositorInsertUbiquitin (UBB Human)
UseTagsEYFP and HAExpressionMammalianMutationPromoterAvailable sinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDule-Tet3.0
Plasmid#174080PurposeTetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.DepositorInsertTet3.0 tRNA synthetase and tRNA
UseTagsExpressionBacterialMutationL270G, Y271L, N311G, C313APromoterAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
β3-integrin-YFP
Plasmid#26653DepositorInsertβ3-integrin (ITGB3 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-ASAP1
Plasmid#52519PurposeFor mammalian expression of the fluorescent voltage sensor ASAP1DepositorInsertAccelerated Sensor of Action Potentials 1
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBad-LgBiT-PhoCl1-SmBiT-MBP
Plasmid#164034Purposeluciferase-based PhoCl screening constructDepositorInsertLgBiT-PhoCl1-SmBiT-MBP
UseTagsHis tag, T7 tag, Xpress TagExpressionBacterialMutationPromoteraraBad promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
mAzurite-16xRhoBAST
Plasmid#196347PurposeExpression of mAzurite mRNA with 16xRhoBAST in 3'UTRDepositorInsertmAzurite-16xRhoBAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB3-mScarlet3_N1
Plasmid#189770PurposeConstruct for labelling microtubule plus ends in mammalian cells with EB3-mScarlet3DepositorInsertEB3-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac CMV-GFP-FRT
Plasmid#92078Purposeproduction of transgenic animal and recombination using flipaseDepositorInsertCMV-eGFP and TK-Neo between FRT3 and FRT
UsePiggybacTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFS478
Plasmid#89066PurposeKanMX Z3EV promoter cassetteDepositorInsertZ3EV promoter cassette
UseYeast integration vectorTagsExpressionMutationyeast Gal1 promoter modified with multiple Zif268…PromoterZ3EV promoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCyterm-mScarlet-I3_N1
Plasmid#189777PurposeConstruct for testing oligimerization of mScarlet-I3 in mammalian cells by OSER assayDepositorInsertCyterm-mScarlet-I3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO ChETA-EYFP
Plasmid#26968PurposeAAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activationDepositorHas ServiceAAV1, AAV5, and AAV9InserthChR2(E123T/H134R)-EYFP
UseAAVTagsEYFPExpressionMammalianMutationE123T, H134RPromoterEF1alphaAvailable sinceJan. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pSF464
Plasmid#167672PurposeIntermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-2
Plasmid#175362PurposeG418R backbone for recombinant protein overexpression with Drosophila ExpreS2 PlatformDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only