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Plasmid#73717PurposeC. elegans germline CRE expression vectorDepositorInsert2xNLS-CRE
UseCre/LoxTagsExpressionWormMutationPromoterPeft-3Available sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA
Plasmid#191528PurposeVector for tandem expression of SLBP 3'UTR G1 sgRNA in combination with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate SLBP endogenous tagging by marker free coselection using ouabainDepositorInsertSLBP 3'UTR G1 sgRNA + ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via hdr using ouabainTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
vHB66
Plasmid#193456PurposeVector for expression of second copy genes in pandoravirus/mollivirusDepositorTypeEmpty backboneUsePandoravirus/mollivirus expressionTagsExpressionMutationPromoterpneo650Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAW188
Plasmid#113178PurposeE. coli expression vector for untagged E. coli Cas1 R131A + Cas2DepositorInsertsCas1
Cas2
UseTagsExpressionBacterialMutationR131APromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW167
Plasmid#113187PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 R40EDepositorInsertsCas1
Cas2
UseTagsExpressionBacterialMutationR40EPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW156
Plasmid#113185PurposeE. coli expression vector for untagged E. coli Cas1 K259E + Cas2DepositorInsertsCas1
Cas2
UseTagsExpressionBacterialMutationK259EPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW186
Plasmid#113184PurposeE. coli expression vector for untagged E. coli Cas1 R163A + Cas2DepositorInsertsCas1
Cas2
UseTagsExpressionBacterialMutationR163APromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW151
Plasmid#113183PurposeE. coli expression vector for untagged E. coli Cas1 R146A + Cas2DepositorInsertsCas1
Cas2
UseTagsExpressionBacterialMutationR146APromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW150
Plasmid#113182PurposeE. coli expression vector for untagged E. coli Cas1 S143A + Cas2DepositorInsertsCas1
Cas2
UseTagsExpressionBacterialMutationS143APromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW174
Plasmid#113181PurposeE. coli expression vector for untagged E. coli Cas1 R138A + Cas2DepositorInsertsCas1
Cas2
UseTagsExpressionBacterialMutationR138APromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW189
Plasmid#113180PurposeE. coli expression vector for untagged E. coli Cas1 Q136A + Cas2DepositorInsertsCas1
Cas2
UseTagsExpressionBacterialMutationQ136APromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW185
Plasmid#113179PurposeE. coli expression vector for untagged E. coli Cas1 R132A + Cas2DepositorInsertsCas1
Cas2
UseTagsExpressionBacterialMutationR132APromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW184
Plasmid#113176PurposeE. coli expression vector for untagged E. coli Cas1 R112A + Cas2DepositorInsertsCas1
Cas2
UseTagsExpressionBacterialMutationR112APromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW152
Plasmid#113175PurposeE. coli expression vector for untagged E. coli Cas1 K12E + Cas2DepositorInsertsCas1
Cas2
UseTagsExpressionBacterialMutationK12EPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW158
Plasmid#113177PurposeE. coli expression vector for untagged E. coli Cas1 R112E + Cas2DepositorInsertsCas1
Cas2
UseTagsExpressionBacterialMutationR112EPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpCas9_sgRNA_expression_in_pBluescript
Plasmid#122089PurposeU6 driven SpCas9 sgRNA expression vector for cloning own guidesDepositorInsertSpCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
NmCas9_sgRNA_expression_in_pBluescript
Plasmid#122091PurposeU6 driven NmCas9 sgRNA expression vector for cloning own guidesDepositorInsertNmCas9 tracrRNA (NEWENTRY S. pyogenes, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ220 (AsCpf1)
Plasmid#86208PurposeAsCpf1 Gateway entry plasmidDepositorInsertAsCpf1
UseGateway compatible cpf1 vectorsTags5' and 3' NLSExpressionPlantMutationPromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
CROP-sgRNA-MS2
Plasmid#153457PurposeCROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffoldDepositorInsertsgRNA empty backbone
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST1374-NLS-flag-linker-Cas9
Plasmid#44758DepositorInsertHuman codon optimized Cas9
UseCRISPRTags32 amino acid linker, Flag, and NLSExpressionMammalianMutationcodon-optimizedPromoterCMVAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only