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Plasmid#186534PurposeLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actinDepositorInsertLSSmGFP
UseTagsLifeActExpressionMammalianMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
eMagA-mCherry-Mito
Plasmid#162251PurposeBlue-light dependent heterodimerization systemDepositorInserteMagA-mCherry-OMP25
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lys-eMagB-iRFP
Plasmid#162249PurposeBlue-light dependent heterodimerization systemDepositorInsertp18-eMagB-iRFP
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEIAV-SIN6.1 CBLacZ-LucWm1A
Plasmid#44167DepositorInsertsLacZ
firefly luciferase
UseLentiviral and LuciferaseTagsExpressionMutationPromoterCB, CMV enhancer/chicken beta-actin promoterAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAV0782
Plasmid#133514PurposeExpression of sfGFP tagged LifeAct probe to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LifeAct-Venus
Plasmid#87613PurposeActin-targeted Venus (YFP).DepositorInsertLifeAct-Venus
UseTags17-aa sequence from Abp140 (aka "Lifeact&quo…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
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mTFP1-Lifeact-7
Plasmid#54749PurposeLocalization: Actin, Excitation: 462, Emission: 492DepositorTypeEmpty backboneUseTagsmTFP1ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pAV0783
Plasmid#133515PurposeExpression of mCherry tagged LifeAct probe to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mouse PKA-Calpha-mEGFP
Plasmid#45528DepositorInsertPKA catalytic subunit alpha tagged by mEGFP
UseWith chicken beta actin promoter and wpreTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAV0771
Plasmid#133513PurposeExpression of mTagBFP2 tagged CHD domain to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-3mTagBFP2-CHDRng2-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCAGGS-rat PKA-RIIbeta-mEGFP
Plasmid#45530DepositorInsertPKA regulatory subunit RII beta tagged by mEGFP
UseWith chicken beta actin promoter and wpreTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
VinculinTS-A50I/I997A
Plasmid#111829PurposeVinculin tension sensor with point mutations affecting ability to bind talin and actinDepositorInsertVinculinTS (I997A)
UseTagsExpressionMammalianMutationchanged vinculin alanine 50 to isoleucine and iso…PromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOC4 Actb-mRuby3 KI
Plasmid#183437PurposeFlpON knock-in for mRuby3-beta-actin (amino acid position: before start codon)DepositorInsertgRNA and mRuby3 donor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-LplA(AILR,36)-NRX3b
Plasmid#43919DepositorInsertAILR-LplA36-NRX3b (lplA Synthetic, Human)
UseTags2XHAExpressionMammalianMutationPromoterChicken beta actinAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
mPapaya1-Lifeact-7
Plasmid#54693PurposeLocalization: Actin, Excitation: 530, Emission: 541DepositorTypeEmpty backboneUseTagsmPapaya1-LifeactExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGs-Hoxd10
Plasmid#45721DepositorInsertHoxd10 (HOXD10 Chicken)
UseTagsExpressionMutationPromoterCMV / b-actinAvailable sinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only