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RGS-6xHis-mRFP-pcDNA3.1-

Plasmid

#52516

Purpose

expresses mRFP in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

mRFP

Use

Tags

RGS-6xHis

Expression

Mammalian

Mutation

engineered variant of tetrameric red fluorescent …

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pT2KXIGdeltaIn-MCS-birA-tagRFP

Plasmid

#58378

Purpose

tol2 transgenesis for biotin based TRAP - BirA ligase

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

birA tagRFP

Use

Tags

tagRFP

Expression

Mutation

Promoter

Available since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-Atoh1wt-IRES-RFP

Plasmid

#33334

Depositor

Charles Sherr, Martine Roussel

Article

Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pPRISM-Fuse-gcry-mRFP

Plasmid

#117784

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-FUSN-miRFP670-Cry2WT

Plasmid

#122441

Purpose

Expresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L

Plasmid

#52478

Purpose

Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

EGFP
nuclear mTagBFP
Synaptophysin-TagRFP-T

Use

Rabies virus

Tags

TagRFP-T

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-GBP-dCas9-mRFP

Plasmid

#102933

Purpose

Expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)

Depositor

Sebastian Bultmann

Article

Anton et al Nucleus. 2017 May 4;8(3):279-286. doi: 1

Insert

dCas9

Use

CRISPR

Tags

GBP (GFP binding protein) and mRFP

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only
pPRISM-Cre-cmlc2-mRFP

Plasmid

#117793

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR CMVie-Lifeact-iRFP670 L1-R5

Plasmid

#84390

Purpose

Multisite Gateway entry vector for expression of iRFP670-tagged Lifeact in cells including neurons

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Gateway

Tags

iRFP670

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-SRGAP2A-FBAR-mRFP

Plasmid

#168502

Purpose

Expression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.

Depositor

Franck Polleux

Article

Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

Insert

SRGAP2A-FBAR (SRGAP2 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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PacrAB-rfp + PmutS-yfp

Plasmid

#121442

Purpose

plasmid reporting the expression from the acrAB and mutS promoters

Depositor

Mary Dunlop

Article

El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

Inserts

PmutS-yfp
PacrAB-rfp

Use

Synthetic Biology; Pbb vector

Tags

Expression

Bacterial

Mutation

Promoter

PacrAB and PmutS

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCVL iRFP-T2A-Trex2

Plasmid

#50418

Purpose

Coexpresses Trex2 and iRFP in mammalian cells.

Depositor

Andrew Scharenberg

Inserts

iRFP
Trex2

Use

Lentiviral

Tags

T2A

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pPHR-iRFP713-NLS-VP16

Plasmid

#103823

Purpose

Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-iRFP713-VP16 (CRY2 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

VP16: G126T (silent, G42G)

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Pdx1-RFP hygro (DM#236)

Plasmid

#15110

Depositor

Douglas Melton

Article

Rajagopal et al Science. 2003 Jan 17. 299(5605):363.

Insert

Pdx1 promoter (Pdx1 Mouse)

Use

Tags

RFP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pFA6a-GBP-RFP-hphMX6

Plasmid

#105161

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-hphMX6

Use

Tags

GBP-RFP

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-tdiRFP670

Plasmid

#82621

Purpose

Expresses NLS-tdiRFP670 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

NLS-tdiRFP670

Use

Piggybac vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pExodus iRFP-T2A-Trex2

Plasmid

#50419

Purpose

Coexpresses Trex2 and iRFP in mammalian cells.

Depositor

Andrew Scharenberg

Inserts

iRFP
Trex2

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pLV.U6gT28.13.EFS-NS.H2B-RFP

Plasmid

#170388

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS-NS

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-E1.1-NRSE-RFP

Plasmid

#22929

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

E1.1 binding site from Scardigli et al. 2003, with neuron-restrictive silencing element, with minimal CMV

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pRFP shRNA-resistant TrkB WT

Plasmid

#72714

Purpose

Mammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

TrkB

Use

Tags

RFP

Expression

Mammalian

Mutation

shRNA-resistant TrkB (T300C, T301A, C302G, C306A,…

Promoter

CMV

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only