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Plasmid#176676PurposeExpression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only
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AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor
Plasmid#178087PurposeHDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertmScarlet-I mTOR signaling indicator (TOSI)
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pShoHELIX-sgRNA_entry (BO3)
Plasmid#181784PurposeExpresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH615
Plasmid#169604PurposeTier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (A1-pA::PCREm4-Nluc-pA::PmPGK1-Fluc-pA).DepositorInsertPPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
UseTagsExpressionMammalianMutationPromoterPCREm4 / PPGKAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL0721 (pDonor_L1-105)
Plasmid#130645PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened left end. Total transposon size = 933 bp.DepositorInsertVchCAST donor DNA (short L)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBCV
Plasmid#62659PurposeMammalian expression of FLPo recombinase from the broadly active CAG promoter/enhancerDepositorInsertFLPo
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJP72
Plasmid#176479PurposeExpresses mScarlet under control of the Capsaspora EF1 promoter and GenR under control of the Capsaspora actin promoterDepositorInsertsactin > GenR
EF1 > mScarlet
UseCapsaspora owczarzaki expressionTagsExpressionMutationPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL0715 (pDonor_R1-57)
Plasmid#130644PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened right end. Total transposon size = 910 bp.DepositorInsertVchCAST donor DNA (short R)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1 Insulator ObLiGaRe/EPB71
Plasmid#90018PurposeDonor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locusDepositorInsertMCS flanked by insulator and inverted ZFN binding sites
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTW080
Plasmid#115928PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/30 SGP NA (No Aptamer)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGE497
Plasmid#153249PurposeM6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
BC1455-LMNA 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)
Plasmid#164042PurposeCRISPR donor plasmid to insert mCherry-harboring CRISPR-TagV2 into the N-terminus of human LMNA geneDepositorInsertmCherry-CRISPR-TagV2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pDGE660
Plasmid#153258PurposeM4E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAT9675-CBE
Plasmid#163007Purposeplasmid expressing CBEDepositorInsertCBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aalb_726
Plasmid#176661PurposeExpression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP86
Plasmid#83543PurposeUsed to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.DepositorInsertRPL4A promoter-yEGFP
UseTagsExpressionYeastMutationPromoterRPL4AAvailable sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry
Plasmid#177183Purposeplasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry markerDepositorInsertsgRNA targeting the PEAR-GFP plasmid
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aaeg_763
Plasmid#176658PurposeExpression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pAT9993-Hypa-ABE
Plasmid#163001Purposeplasmid expressing Hypa-ABEDepositorInsertHypa-ABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9676-ABE
Plasmid#162997Purposeplasmid expressing ABEDepositorInsertABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-BvDODAα1
Plasmid#162528PurposeBeta vulgaris 4,5-DOPA dioxygenase alpha 1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.DepositorInsertDODAα1
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJBL703
Plasmid#140373PurposeExpresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoterDepositorInsertT7-2BP-tetO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFP84
Plasmid#83542PurposeUsed to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.DepositorInsertSSA1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterSSA1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pIK137
Plasmid#65632PurposeExpresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragmentDepositorInsertPeft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment
UseTagsExpressionWormMutationPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pAP2050-2
Plasmid#105243Purposemammalian GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linkerDepositorInsertGFP11 with extra-sequence (GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT1100
Plasmid#8618DepositorInsertribonuclease Sa3, barstar
UseTagsphoA signal sequence to RNase Sa3ExpressionBacterialMutationIn addition to the substitution of the barnase ge…PromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAP2042-1
Plasmid#105244Purposemammalian eGFP with TEV::3xFlag::Ollas::Myc::V5::HAtag::other linkerDepositorInserteGFP with extra-sequence (TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker::eGFP)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS1011
Plasmid#63783PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection markerDepositorInsertStrep-tag II followed by TRP1 selection marker
UseE. coli cloning vectorTagsStrep-tag IIExpressionMutationPromoternoneAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTW088
Plasmid#115936Purpose3'UTR insert for replicon MoCloDepositorInsertLvl0 3'UTR No E1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW094
Plasmid#115937Purpose3'UTR insert for replicon MoCloDepositorInsertLvl0 E1-3'UTR End
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
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mCherry-pCAG-donor(R2Tg)-GFP(intron)
Plasmid#223248PurposeMammalian expression of the R2Tg RNA donorDepositorInsertR2Tg donor
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-R2Tg-3xFlag
Plasmid#223250PurposeMammalian expression of C-terminally Flag-tagged R2Tg proteinDepositorInsertR2Tg protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Xlone Puro SNAP-eGFP-BASU
Plasmid#218452Purposeexpresses the SNAP-eGFP-BASU fusion protein via stable PiggyBac integrationDepositorInsertSNAP-eGFP-BASU
UseTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA335
Plasmid#216069PurposeFragmid fragment: (2A Selection) Stop; IRES2; Start; HygroR; StopDepositorInsertStop; IRES2_v1; Start; HygroR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
p944-SWITCH-OVER insert: EF1a-Puro
Plasmid#217891Purposeinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p931-SWITCH-OVER insert: EF1a-Blasti
Plasmid#217890Purposeinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p1071-Lenti-Single loxP
Plasmid#217892PurposeLentiviral vector, entry vector for Lentiviral Switch-OVERDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p223-Pulse-Switch
Plasmid#217889PurposeLentiviral Pulse-Switch vector for sgRNA expression, Cas9 and sgRNA expression are mutual exclusive; blastiRDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only