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pET-CjCas9

Plasmid

#89754

Purpose

Expression of CjCas9 with His tag in E.coli

Depositor

Seokjoong Kim

Article

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

CjCas9

Use

CRISPR

Tags

6XHis, HA, and SV40 NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1-CAG-hrGFP

Plasmid

#52344

Purpose

AAVS1 donor vector for constitutive gene expression

Depositor

Su-Chun Zhang

Article

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

hrGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Wt1

Plasmid

#41082

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Wt1 (-KTS alternative isoform) (Wt1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO + minimal CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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Arp3-pmCherryC1

Plasmid

#27682

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Arp3 (ACTR3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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GFAP-Cre

Plasmid

#24704

Depositor

Michael Sofroniew

Article

Bush et al Neuron. 1999 Jun . 23(2):297-308.

Insert

Cre

Use

Tags

Expression

Mammalian

Mutation

Promoter

GFAP (glial fibrillary acidic protein)

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pSpot-Tag-GFP-GPI

Plasmid

#117061

Purpose

For mammalian expression of GPI-anchored GFP with N-terminal Spot-Tag

Depositor

Felix Hartlepp

Insert

GPI-anchored GFP

Use

Tags

GPI-anchor and Spot-Tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET28HIS-hAPE1

Plasmid

#70757

Purpose

bacterial expression of human APE1

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

APEX1 (APEX1 Human)

Use

Tags

6-HIS

Expression

Bacterial

Mutation

Promoter

T7/lac operon

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pSMPP-mCherry-mTrim21

Plasmid

#104971

Purpose

Lentiviral Transfer Plasmid with mCherry-tagged mouse Trim21

Depositor

Leo James

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Tripartite Motif containing 21 (Trim21 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

SFFV

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec23A

Plasmid

#66609

Purpose

GFP fusion of Sec23A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec23A (SEC23A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pIRES2-hPAC-FLAG

Plasmid

#129494

Purpose

Expresses human TMEM206 tagged at the C-terminus with FLAG

Depositor

Zhaozhu Qiu

Article

Yang et al Science. 2019 Apr 26;364(6438):395-399.

Insert

human PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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dCas9-10xSunTag(22aa)

Plasmid

#215745

Purpose

Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

dCas9-10xSunTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

chicken beta-actin promoter

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NCAM1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156948

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NCAM1 (NCAM1 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1 GFP shRNA

Plasmid

#30323

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

GFP shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2011

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(P691S)-FLAG

Plasmid

#164973

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

P691S

Promoter

EF1a

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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FRZBA-c400

Plasmid

#205109

Purpose

FRZB protein expression

Depositor

Paul Brennan

Insert

FRZB (FRZB Human)

Use

Tags

AviTag-6His and PTPRSsp

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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MBX

Plasmid

#64122

Purpose

a modified episomal (EBNA1/OriP) vector expressing human BCL2L1 (BCL-xL) gene

Depositor

Linzhao Cheng

Article

Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

Insert

BCL2L1 (BCL2L1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAM1

Plasmid

#51401

Purpose

Expresses truncated version of NLL-MetRS from endogenous MetG promoter

Depositor

David Tirrell

Article

Mahdavi et al Proc Natl Acad Sci U S A. 2014 Jan 7;111

Insert

MetRS (metG E coli)

Use

Tags

Expression

Mutation

L13N, Y260L, H301L; Please note that a truncated …

Promoter

MetG

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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SpdCas9 KRAB

Plasmid

#188499

Purpose

Expresses FLAG tagged SpdCas9 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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AICSDP-36: AAVS1-mEGFP (PGK)

Plasmid

#114404

Purpose

Homology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP

Depositor

Allen Institute for Cell Science

Insert

AAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)

Use

CRISPR; Donor template

Tags

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

Sept. 4, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEBG‐AMPK α1(1‐312)

Plasmid

#27632

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

AMPK α1(1‐312) (Prkaa1 Rat)

Use

Tags

GST

Expression

Mammalian

Mutation

Constitutively  active  by  truncation  after  am…

Promoter

Available since

May 18, 2011

Availability

Academic Institutions and Nonprofits only

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