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pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR

Plasmid

#210523

Purpose

AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

GFP-KASH
U6-gRNA-directcapture

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

Plasmid

#83842

Purpose

Fluorescent probe for S/G2 cell cycle transition and G2/M transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Human, Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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sABE v3.22_N

Plasmid

#209553

Purpose

pCMV-bpSV40 NLS-TadA8e(2-76)-2xFRB-BGHpA

Depositor

Xue Gao

Article

Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

Insert

TadA-8e(2-76)-2xFRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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sABE v3.22_C

Plasmid

#209554

Purpose

pCMV-SV40_NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A)-SV40_NLS-BGHpA

Depositor

Xue Gao

Article

Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

Insert

2xFKBP-TadA-8e(77-167)-SpCas9(D10A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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plenti hSyn PKM1

Plasmid

#220920

Purpose

Neuronal specific expression of PKM1

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PKM1 (PKM Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJAM1.19_PB-CIT-PGK-Blast

Plasmid

#205564

Purpose

Express human CIT

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

CIT (CIT Human)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMOS023E: Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

Plasmid

#163077

Purpose

Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Peredox

Use

Gateway entry vector

Tags

mCherry

Expression

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-IDH1

Plasmid

#62906

Purpose

Expresses wildtype IDH1 in mammalian cells

Depositor

Yue Xiong

Article

Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

Insert

isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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TL026_AAV_hSyn:SEP-GluA1

Plasmid

#192519

Purpose

Expression of AMPA GluA1 with N terminal super ecliptic phluorin (SEP) fusion

Depositor

Adam Cohen

Article

Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

Insert

SEP-GluA1

Use

AAV and Cre/Lox

Tags

super ecliptic phluorin

Expression

Mutation

Promoter

hSyn

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-sL7-Cre-HA-WPRE

Plasmid

#204488

Purpose

For production of driver AAV virus for Purkinje cell-specific expression

Depositor

Samuel Wang

Article

Chen et al Nature. 2022 May;605(7911):722-727. doi:

Insert

Cre

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

sL7

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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