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Plasmid#71136PurposeExpression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to phenylalanine; additional…PromoterT7Available sinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK175
Plasmid#71132PurposeExpression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationadditional N-terminal methionine encoded by this …PromoterT7Available sinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-RpS12-Gateway
Plasmid#71508PurposeflySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the RpS12 core promoterDepositorTypeEmpty backboneUseFlystarr-seq validation vectorTagsluciferaseExpressionInsectMutationPromoterAvailable sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-KQ
Plasmid#64982PurposeExpresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108QPromoterAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrG_B1-DK
Plasmid#117981PurposeSdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B1
UseTagsHIS, sortase, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrG_B2-DK
Plasmid#117982PurposeSdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B2
UseTagsHIS, sortase, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pLV Myc-emerin
Plasmid#175101PurposeLentiviral expression of Myc-tagged mouse EmdDepositorInsertEmd (Emd Mouse)
UseLentiviralTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-EGFP
Plasmid#75159PurposeDerived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-SaCas9
Plasmid#178901PurposeSaCas9 (Cas9 from Staphylococcus aureus)DepositorInsertSaCas9
UseTagsHis6-Tag, bipartite nuclear localization signal f…ExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-RFP657
Plasmid#75162PurposeDerived from LentiCRISPR v1 but expresses RFP657 instead of puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsRFP657ExpressionMammalianMutationPromoterAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pssAAV_Seq17_CMV_bglobin_3NLS_Venus_WPRE_polyA
Plasmid#220224PurposeAAV-STARR-seq validation control vector with VenusDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-tagBFP
Plasmid#75160PurposeDerived from LentiCRISPR v1 but expresses tagBFP instead of puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagstagBFPExpressionMammalianMutationPromoterAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Ls-AChBP
Plasmid#63126PurposeExpression of Lymnaea stagnalis AChBPDepositorInsertAChBP
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHD157
Plasmid#84033PurposeVector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the fabp10 promoter and Venus under the crystalin promoter in zebrafishDepositorInsertsCre
Venus
UseCre/Lox; Zebrafish transgenesisTagsmCherryExpressionMutationPromoterCrystalin and fabp10Available sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBstSK+IRES-V5-IPpoI
Plasmid#206321PurposeA plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoIDepositorInsertIRES element-V5 epitope tag- IPpoI open reading frame
UseMrna productionTagsV5-IPpoI fusion proteinExpressionMutationPromoterAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero9
Plasmid#187927PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero9DepositorInsertHero9
UseTagsFlag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVk7-33G1-BioLHis
Plasmid#58949Purposebackbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tagDepositorTypeEmpty backboneUseTags6His tag and biotinylation peptideExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVk7-33G1-Flag
Plasmid#58950Purposebackbone for mouse recombinant antibody with added C-terminal FLAG tagDepositorTypeEmpty backboneUseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJL1-aadA
Plasmid#117050PurposeIn vitro expression of aadA (streptomycin resistance gene) from the T7 promoterDepositorInsertaadA
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AUA-IRES-mCherry
Plasmid#222116PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralTagsExpressionMutationAUA start codonPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only