-
Plasmid#67878PurposeThe human dysferlin cDNA was codon optimized in an attempt to increase Dysferlin production in human cells.DepositorInsertcodon optimized Dysferlin
UseUnspecifiedTagsNoExpressionMutationPromoterNo PromoterAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
TagRFP-T-VAPB(1- 218)- eMagB
Plasmid#162255PurposeBlue-light dependent heterodimerization systemDepositorInsertTagRFP-T-VAPB(1- 218)- eMagB
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOIMars1CyN
Plasmid#75221PurposeMammalian cell surface expression of fluorogen activating protein Mars1CyDepositorInsertss-HA-Mars1Cy-CD80(237-306)
UseTagsHA epitopeExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pP2AeGFP-Mars1Cy
Plasmid#75223PurposeMammalian intracellular expression of fluorogen activating protein Mars1Cy and eGFPDepositorInsertEGFP-p2a-Mars1Cy
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET11a-DAAT-V33G/T242G
Plasmid#159278PurposeAminotransferase variant with near-native catalytic efficiency toward D-tryptophanDepositorInsertEngineered d-alanine aminotransferase
UseTags6x His tagExpressionBacterialMutationValine 33 and threonine 242 have been mutated to …PromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTX1-EV71-SL2
Plasmid#126043PurposeIn-vitro-transcription of Enterovirus 71 5UTR-SL2 RNA in NMR tube for "Systems NMR" analysisDepositorInsertEnterovirus 71 5'UTR SL2
UseTagsExpressionMutationPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTX1-SMN1-exon7-ESE1
Plasmid#126041PurposeIn-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysisDepositorInsertSMN1 exon7 ESE1
UseTagsExpressionMutationPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenCMV-PMMars1CyGFP
Plasmid#78315PurposeLentiviral transfer vector encoding Mars1Cy-GFP membrane-spanning proteinDepositorInsertMars1Cy-TMD-GFP
UseLentiviralTagsHA epitopeExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-28b(+)/ADK C77S
Plasmid#201409PurposeOverexpresses Escherichia coli adenylate kinase C77SDepositorInsertEscherichia coli adenylate kinase C77S (adk Escherichia coli)
UseTagsExpressionBacterialMutationchanged cysteine 77 to serinePromoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2911
Plasmid#161764PurposeMODULE C plasmid compatible with the Voytas Plant Genome Engineering Tool kit with an Agrobacterium promoter rolD expressing the TREX2 exonuclease.DepositorInsertrolD:TREX2
UseTagsExpressionBacterialMutationPromoterrolDAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
VPS13C^halo∆1235-1748
Plasmid#187297PurposeExpress VPS13C truncation mutant in mammalian cellsDepositorInsertVPS13C truncation mutant (VPS13C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMET7-GAG-eDHFR
Plasmid#194635PurposeMammalian expression of the eDHFR control bait in VirotrapDepositorInsertp55 GAG
UseTagsecDHFRExpressionMammalianMutationPromoterSRalphaAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH3971
Plasmid#177736PurposePrgef-1 GCaMP6::3xNLS::mNeptuneDepositorInsertGCaMP
UseTagsmNeptuneExpressionWormMutationPromoterPrgef-1Available sinceDec. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTX1-SMN2-exon7-ESE1
Plasmid#126042PurposeIn-vitro-transcription of SMN2-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysisDepositorInsertSMN2 exon7 ESE1
UseTagsExpressionMutationPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTH861-gst-optec P210T
Plasmid#89595PurposePlasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase P210T mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged proline 210 to threoninePromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH846-gst-dissc
Plasmid#96907PurposePlasmid for yeast expression of GST encoded by non-preferred codonsDepositorArticleInsertyeast codon dis-optimised glutathione-S transferase
UseTags8xhistidineExpressionYeastMutationPromoterTDH3Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH860-gst-optec A180Q
Plasmid#89594PurposePlasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged alanine 180 to glutaminePromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH858-gst-optec V156E
Plasmid#89592PurposePlasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase V156E mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged valine 156 to glutamic acidPromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH859-gst-optec Y172M
Plasmid#89593PurposePlasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged tyrosine 172 to methioninePromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV(CAT)T7-SB100
Plasmid#34879DepositorInsertSB100X transposase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only