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Plasmid#218083PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN0059-C
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNPFLAGH_BN006V-4
Plasmid#218080PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN006V-4
UseTagsFLAG-His and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLC025 - pL0_StopCodon (CT)
Plasmid#203905PurposeMoClo compatible L0 plasmid containing a stop codon. To allow for proper fusion with CDS1ns L0 plasmids, this plasmid adds an additional glycine and serine to the C-terminal end of a CDS and then adds a stop codon to terminate translation.DepositorInsertStop codon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN381 - pTRV2-CHLH
Plasmid#203886PurposeViral silencing plasmid targeting the CHLH gene from C. roseus. For use as a positive control for VIGS experiments.DepositorInsertCHLH fragment
UseRNAiTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN1243 - pL0_pT3O (pro + 5U)
Plasmid#203891PurposeT3O promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsertT3O promoter and 5'UTR
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN1198 - pL0_pD4H (pro + 5U)
Plasmid#203894PurposeD4H promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsertD4H promoter and 5'UTR
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry
Plasmid#202090PurposeNeuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutationsDepositorInsertdCas9-Set2(R195G, C201A)
UseTags3x FLAG and mCherryExpressionMammalianMutationR195G and C201A abolish catalytic activityPromoterhuman SynapsinAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIALD2HMGr
Plasmid#185867PurposeKnocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiaeDepositorInsertALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2F3-P229L-pET151
Plasmid#177879PurposeExpresses mouse Talin 1 F2F3-P229L domains in bacterial cellsDepositorInsertmouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Leucine (P229L)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2F3-P229S-pET151
Plasmid#177878PurposeExpresses mouse Talin 1 F2F3-P229S domains in bacterial cellsDepositorInsertmouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Serine (P229S)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22gCfpUbiG4FER4P2A(#249)
Plasmid#184070Purposecyclofen-inducible GAL4 activation in zebrafish permanent transgenicDepositorInsertGal4bdFF-ERT2-P2A
UseZebrafish transgenesis (blue eyes)TagsExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2X-ORF24-NTD-3L_A
Plasmid#138466PurposeExpresses MBP-tagged ORF24-NTD 3L_A in bacteriaDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsMBPExpressionBacterialMutationAmino acids 1-201; amino acids L73-75 mutated to …PromotertacAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQLink-GST-2xCTD
Plasmid#138470PurposeExpresses GST-tagged 2xCTD heptapeptide repeat in bacteriaDepositorInsertRpb1 (POLR2A Human)
UseTagsGSTExpressionBacterialMutation2x CTD repeatPromoterT5Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 L73A_L75A-3xFLAG
Plasmid#138429PurposeExpresses full-length ORF24 L73A/L75A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acids L73, L75 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 L74A_L75A-3xFLAG
Plasmid#138430PurposeExpresses full-length ORF24 L74A/L75A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acids L74, L75 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 R68A-3xFLAG
Plasmid#138431PurposeExpresses full-length ORF24 R68A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acid R68 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 R72A-3xFLAG
Plasmid#138432PurposeExpresses full-length ORF24 R72A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acid R72 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 L73A_L74A-3xFLAG
Plasmid#138433PurposeExpresses full-length ORF24 L73A/L74A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acids L73, L74 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-UL87 3L_A-2xStrep
Plasmid#138437PurposeExpresses full-length UL87 3L_A with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertUL87 (UL87 HCMV (HHV-5))
UseTagsStrepExpressionMammalianMutationAmino acids L90-L92 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-BcRF1 3L_A-2xStrep
Plasmid#138439PurposeExpresses full-length BcRF1 3L_A with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertBcRF1 (BcRF1 EBV (HHV-4))
UseTagsStrepExpressionMammalianMutationAmino acids L64-L66 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only