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Showing: 1381 - 1400 of 15679 results

  1. pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

    Plasmid
    #215014
    Purpose
    Express an autophagy (LC3) reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    MAP1LC3B (Map1lc3b Rat)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215014-plasmid-map-sequence-id-429267

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  3. p663-UBC-TurboID-V5-eGFP_IDG-K

    Plasmid
    #135264
    Purpose
    Gateway destination clone of eGFP (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-eGFP
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135264-plasmid-map-sequence-id-268064

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  4. pPuro3.1(+)_Strep-Lrrk2-Myc

    Plasmid
    #161583
    Purpose
    expression of full-length mouse Lrrk2 with C-terminal Myc tag
    Depositor
    Maik Hintze
    Insert
    Lrrk2 (Lrrk2 Mouse)
    Use
    Tags
    Myc tag and StrepII
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    161583-plasmid-map-sequence-id-318587

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  7. pLenti-CMV-PtenG129E-Puro

    Plasmid
    #135678
    Purpose
    Constitutive expression (cMV promoter) of G129E mutant Pten cDNA, Puromycin selection
    Depositor
    Florian Karreth
    Insert
    Pten (Pten Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    G129E
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135678-plasmid-map-sequence-id-327597

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  8. pLenti-CMV-PtenK13E-Puro

    Plasmid
    #135680
    Purpose
    Constitutive expression (cMV promoter) of K13E mutant Pten cDNA, Puromycin selection
    Depositor
    Florian Karreth
    Insert
    Pten (Pten Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    K13E
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135680-plasmid-map-sequence-id-327598

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  9. pLenti-CMV-PtenY138L-Puro

    Plasmid
    #135679
    Purpose
    Constitutive expression (cMV promoter) of Y138L mutant Pten cDNA, Puromycin selection
    Depositor
    Florian Karreth
    Insert
    Pten (Pten Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Y138L
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135679-plasmid-map-sequence-id-327594

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  10. p663-UBC-TurboID-V5-dsRed_IDG-K

    Plasmid
    #135261
    Purpose
    Gateway destination clone of dsRed (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-dsRed
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135261-plasmid-map-sequence-id-268068

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  11. p667-UBC-GFP-V5-miniTurbo_IDG-K

    Plasmid
    #135240
    Purpose
    Gateway destination clone of GFP (as control) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    GFP-V5-miniTurbo
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135240-plasmid-map-sequence-id-268088

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  12. p667-UBC-hcRed-V5-miniTurbo_IDG-K

    Plasmid
    #135237
    Purpose
    Gateway destination clone of hcRed (as control) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    hcRed-V5-miniTurbo
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135237-plasmid-map-sequence-id-311572

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  15. Aiolos ZF2 (AA146-168) in Cilantro 2

    Plasmid
    #74456
    Purpose
    Monitor post-translational degradation of Aiolos ZF2 via EGFP:mCherry ratio (Lentiviral, PGK.AiolosAA146-168-10AAFlexLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Insert
    Aiolos (IKZF3) AA130-189 (IKZF3 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    74456-plasmid-map-sequence-id-140672

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  16. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid
    #174902
    Purpose
    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Y306A, Y384F
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174902-plasmid-map-sequence-id-344602

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  19. PERK 7: PERK.K618A.9E10.pBABE

    Plasmid
    #21820
    Depositor
    David Ron
    Insert
    PERK K618A (Eif2ak3 Mouse)
    Use
    Retroviral
    Tags
    Myc (9E10)
    Expression
    Mammalian
    Mutation
    kinase dead K618A mutant
    Promoter
    Available since
    Oct. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    21820-plasmid-map-sequence-id-10016

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Showing: 1381 - 1400 of 15679 results