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pcDNA3/F-tractin=mStayGold

Plasmid

#212019

Purpose

For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

F-tractin

Use

Tags

mStayGold

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA GFP10-ZIP

Plasmid

#182241

Purpose

Tripartite split-GFP mammalian. Express leucine zipper with GFP10

Depositor

Stéphanie Cabantous

Article

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

GFP10-ZIP

Use

Tags

split-GFP10 tripartite

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA ZIP-GFP11

Plasmid

#182242

Purpose

Tripartite split-GFP mammalian. Express leucine zipper with GFP11

Depositor

Stéphanie Cabantous

Article

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

ZIP-GFP11

Use

Tags

split-GFP11 tripartite

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-NEDD8

Plasmid

#19943

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-ExRai-AMPKAR

Plasmid

#192446

Purpose

Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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3xHA-miniTurbo-NLS_pCDNA3

Plasmid

#107172

Purpose

expresses 3xHA-tagged miniTurbo in the mammalian nucleus

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Use

Tags

Expression

Mammalian

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Pur-mEGFP

Plasmid

#186329

Purpose

Fluorescent reporter system

Depositor

Bertrand Collet

Insert

mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HA-Flag-natT1R2

Plasmid

#113944

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Use

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-839

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-1xHA CARM1 WT

Plasmid

#81118

Purpose

mammalian expression of CARM1 WT

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

CARM1 (Carm1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2016

Availability

Academic Institutions and Nonprofits only
3xHA-TurboID-NLS_pCDNA3

Plasmid

#107171

Purpose

expresses 3xHA-tagged TurboID in the mammalian nucleus

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Use

Tags

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-FbxO5-pcDNA3.1-

Plasmid

#52509

Purpose

expresses human FbxO5 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

FbxO5 (FBXO5 FbxO5, Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNBAS_AA

Plasmid

#148236

Purpose

Mammalian Expression of HsNBAS

Depositor

Elisa Izaurralde

Insert

HsNBAS (NBAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mCherry-CyrCR1

Plasmid

#128768

Purpose

Expresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Cyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v4-kif18b

Plasmid

#74934

Purpose

Translation reporter that lacks PP7 sites in the 3'UTR

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

24xGCN4_v4-kif18b

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_NES_STf

Plasmid

#182001

Purpose

CMV overexpression of SNAP-tag fast strictly in the cytoplasm of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

NES-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ccdB-3xHA

Plasmid

#214986

Purpose

Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xHA tag

Depositor

Wade Harper

Type

Empty backbone

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 APEX2-NES

Plasmid

#49386

Purpose

Expresses APEX2 in the cytosol of mammalian cells

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

Flag - APEX2 - NES

Use

Tags

Flag

Expression

Mammalian

Mutation

K14D, W41F, E112K, A134P on soybean APX; improved…

Promoter

CMV

Available since

Nov. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-DDB1

Plasmid

#19918

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

DDB1 (Damage-specific DNA binding protein 1) (DDB1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ccdB-3xFLAG

Plasmid

#214984

Purpose

Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xFLAG tag

Depositor

Wade Harper

Type

Empty backbone

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA eIF4GI (1-1599)

Plasmid

#45640

Depositor

Nahum Sonenberg

Article

Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

Insert

eIF4GI transcript variant 2 (EIF4G1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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