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pcDNA 3.1 (+) FLAG-Ago2 S387A

Plasmid

#60652

Purpose

Expresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

CMV-FLAG-Ago2 (N-term tag)

Expression

Mutation

S387A mutant

Promoter

CMV

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 S387A

Plasmid

#59905

Purpose

Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

S387A, phospho-null alanine

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 5A

Plasmid

#60651

Purpose

Expresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

mutated five serines to alanines

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 4A

Plasmid

#59907

Purpose

Expresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

mutated four serines to alanines

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-CamKIIa-ChR2-EGFP

Plasmid

#114216

Purpose

Expresses channelrhodopsin-EGFP fusion in CamKIIa+ neurons

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Channelrhodopsin 2

Use

AAV and Mouse Targeting

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CamKIIa 1.3 kb

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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mCer-TG2-YFP

Plasmid

#85471

Purpose

TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET

Depositor

Ray Truant, Jeffrey Keillor

Article

Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371

Insert

hTG2 (TGM2 Human)

Use

Tags

EYFP and mCerulean

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP

Plasmid

#40640

Depositor

Brenda Lilly

Article

Zhao et al J Biol Chem. 2012 May 11;287(20):16111-2

Insert

NICD3 (NOTCH3 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-Caspase-1 (C285A) -CpLxIS

Plasmid

#131355

Purpose

Expresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its C-ternimus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

Caspase-1 (C285A)-CpLxIS (Casp1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Caspase-1 (C285A)

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-Caspase-1 (C285A) -NpLxIS

Plasmid

#131354

Purpose

Expresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its N-ternimus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

Caspase-1 (C285A)-NpLxIS (Casp1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Caspase-1 (C285A)

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Venus-Bad-2A-pEGFP-C1

Plasmid

#166748

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation to 2 positions in the BH3 domain

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bad-2A (BAD Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

2 hydrophobic residues in the BH3 region mutated …

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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Syntaxin4-myc-myc-His

Plasmid

#12377

Depositor

Thomas Weimbs

Article

Low et al Mol Biol Cell. 2006 Feb . 17(2):977-89.

Insert

Syntaxin4 (STX4 Human)

Use

Tags

2xMyc-His

Expression

Mammalian

Mutation

Cloned in modified PCDNA4 vector (double myc)

Promoter

CMV⁄TO

Available since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only

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pEY40

Plasmid

#191044

Purpose

ceh-2 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ceh-2 (ceh-2 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY100

Plasmid

#223447

Purpose

lad-2 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

lad-2 (lad-2 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY134

Plasmid

#223481

Purpose

str-2 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

str-2 (str-2 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEY109

Plasmid

#223456

Purpose

ocr-2 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ocr-2 (ocr-2 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEY111

Plasmid

#223458

Purpose

odr-2 (promB) fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

odr-2(promB) (odr-2 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEY137

Plasmid

#223484

Purpose

tax-2 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

tax-2 (tax-2 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEY90

Plasmid

#191083

Purpose

glr-2 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

glr-2 (glr-2 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEY60

Plasmid

#191057

Purpose

acc-2 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acc-2 (acc-2 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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anxA9-GFP

Plasmid

#107198

Purpose

Expresses human annexin A9-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Goebeler et al FEBS Lett. 2003 Jul 10;546(2-3):359-64.

Insert

annexin A9 (ANXA9 Human)

Use

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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