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Plasmid#183073PurposePlasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repressionDepositorInsertExpressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HCV-Fluc_AC
Plasmid#148447PurposeMammalian Expression of FlucDepositorInsertFluc
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-Rluc-6xbsMS2-random_AC
Plasmid#148431PurposeMammalian Expression of 6xbsMS2-randomDepositorInsert6xbsMS2-random
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-CT-Tagging
Plasmid#165871PurposeTemplate for amplification of EGFP C-terminal tagging cassetteDepositorInsertVector - CT Tagging
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xP3
Plasmid#165779Purpose3xP3 eye driver enhancer element, requires promoter element for functionDepositorInsert3xP3
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDH010
Plasmid#157645PurposesMHT-113 fused non-dimerizing GRX2-C37A mutantDepositorInsertssMHT(1-113)-GRX2_C37A
GRX2_C37A-sMHT(114-230)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-CT-Tagging
Plasmid#165869PurposeTemplate for amplification of mcherry C-terminal tagging cassetteDepositorInsertVector - CT Tagging
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD2
Plasmid#165857Purposeuniversal Vector Domesticator vector v2 used in the integration (domestication) of new vector backbone parts in the GB2.0 cloning system. Uses blue-white screening methodDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NT-Tagging
Plasmid#165868PurposeTemplate for amplification of EGFP N-terminal tagging cassetteDepositorInsertVector - NT Tagging
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSV40L-TN
Plasmid#165827PurposeViral Simian virus 40 Late poly(a) terminator element Compatible with transposon 3' ITRsDepositorInsertSV40L
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGGSx4 CT
Plasmid#165811PurposeFlexible peptide linker of 4 repeats of GGS, for assembly of C-terminal TL5 tags to TL4 partsDepositorInsertGGSx4 CT
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p4xLexAOp-A1
Plasmid#165814PurposeAlpha1 4xLexAOp for assembly of larger LexAOp response elementsDepositorInsert4xLexAOp-A1
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p4xLexAOp-A2
Plasmid#165815PurposeAlpha2 4xLexAOp for assembly of larger LexAOp response elementsDepositorInsert4xLexAOp-A2
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p4xLexAOp-O2
Plasmid#165816PurposeOmega2 4xLexAOp for assembly of larger LexAOp response elementsDepositorInsert4xLexAOp-O2
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p4xLexAOp-O1
Plasmid#165817PurposeOmega1 4xLexAOp for assembly of larger LexAOp response elementsDepositorInsert4xLexAOp-O1
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLexA BD
Plasmid#165821PurposeLexA DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domainDepositorInsertLexA BD
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQFBD
Plasmid#165823PurposeQF DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domainDepositorInsertQFBD
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQFAD
Plasmid#165824PurposeQF activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domainDepositorInsertQFAD
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGGSx4 NT
Plasmid#165810PurposeFlexible peptide linker of 4 repeats of GGS, for assembly of N-terminal TL1 tags to TL3 partsDepositorInsertGGSx4 NT
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Promoterless TP10
Plasmid#165787PurposeDrosphila TP10 poly(a) terminator sequence in promoter grammar, for assembly of no expression control vectorsDepositorInsertPromoterless T10
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only