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Plasmid#215793PurposeCloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
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pcDNA3-MAVS-invitro(KaganD86)
Plasmid#52012PurposeUsed for in vitro expression of the human MAVS CDSDepositorInsertMAVS
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFN-HaloTag-TalinR7R8-AviTag
Plasmid#192047PurposePlasmid expresses human talin R7R8 domain flanked between a HaloTag and a AviTagDepositorInsertTLN1 (TLN1 Human)
UseTagsAviTag and HaloTagExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-Cas9-VQR-GFP_activity_reporter
Plasmid#87156PurposeThis lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).DepositorInsertGFP and sgRNA targeting GFP (NGA restricted)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-SUMO1-AVITAG-GG
Plasmid#215808PurposeCloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage non cleavable N-terminal avitagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMIG-hNFATc2/C(1-686)AVITEV
Plasmid#74054Purposeretroviral expression plasmid for human NFATc2/C (without C-terminal region) with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 AS 1-686 (N terminus deleted), Human)
UseRetroviralTagsAVI-TEVExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-6xHis-AviTag-ALFAtag-eGFP
Plasmid#195854PurposeBacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.DepositorInsert6xHis-AviTag-ALFAtag-eGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-GST-TEV-AVITAG-GG
Plasmid#215809PurposeCloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMIG-hNFATc2/C(RIT)-FLAG-AVITEV
Plasmid#74053Purposeretroviral expression plasmid for human NFATc2/C (with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 Human)
UseRetroviralTagsAVI-TEV and FLAGExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M1A-invitro(KaganE12)
Plasmid#52016PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation M1ADepositorInsertMAVS-M1A
UseTagsExpressionMammalianMutationchanged Met 1 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-G67M-invitro(KaganD100)
Plasmid#52014PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation G67MDepositorInsertMAVS
UseTagsExpressionMammalianMutationChanged Gly 67 to MetPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-L62M-invitro(KaganD98)
Plasmid#52013PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation L62MDepositorInsertMAVS
UseTagsExpressionMammalianMutationChanged Leu 62 to MetPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-E80M-invitro(KaganD99)
Plasmid#52015PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation E80MDepositorInsertMAVS
UseTagsExpressionMammalianMutationChanged Glu 80 to MetPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M142A-invitro(E13)
Plasmid#52017PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation M142ADepositorInsertMAVS-M142A
UseTagsExpressionMammalianMutationchanged Met 142 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCR4-TOPO_L31-FLAG-TEV-AviTag_repair_template
Plasmid#235608PurposeRepair template for inserting FLAG-TEV protease cleavage site-AviTag into the C terminus of mouse Rpl31 via CRISPRDepositorInsertRpl31 C-terminus with FLAG-TEV-AviTag and flanking homology arms
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PDCD1-Fc(DAPA)-AviTag-6xHis
Plasmid#156543PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertPDCD1 (PDCD1 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CD274-Fc(DAPA)-AviTag-6xHis
Plasmid#156660PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertCD274 (CD274 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CD274-COMP5AP-AviTag-9xHis
Plasmid#157224PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertCD274 (CD274 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PDCD1LG2-COMP5AP-AviTag-9xHis
Plasmid#157176PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertPDCD1LG2 (PDCD1LG2 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PDCD1-COMP5AP-AviTag-9xHis
Plasmid#157108PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertPDCD1 (PDCD1 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only