-
-
pUCmini-UGCG-EGFP
Plasmid#225878PurposePlasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertionDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Red Glifon Nega
Plasmid#163117PurposeRed fluorescent protein-based glucose negative control sensor (does not respond to glucose) for mammalian cellsDepositorInsertRed Glifon Nega
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiMutB3GALT5-blast
Plasmid#208381Purposelentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequenceDepositorInsertbeta-1,3-galactosyltransferase 5 (B3GALT5 Human)
UseLentiviralTagsmyc, FLAGExpressionMammalianMutationnucleotide change in PAM targeting sequence nt 51…PromoterEF-1 alpha core promoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
FCIV1-GR SS287AA/S155A
Plasmid#72700PurposeLentiviral expression of GR-2A phosphodeficient mutant (2nd generation)DepositorInsertGR (Nr3c1 Rat)
UseLentiviralTagsIRES-VenusExpressionMammalianMutationSS287AA/S155APromoterubiquitinAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pK18msB
Plasmid#177839PurposePlasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.DepositorTypeEmpty backboneUseBacterial gene replacementTagsExpressionMutationPromoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pk7-GR-GFP
Plasmid#15534DepositorInsertGlucocorticoid receptor (NR3C1 Human)
UseTagsGFPExpressionMammalianMutationThe glucocorticoid receptor was cloned into the X…PromoterAvailable sinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
lentiMutB3GALT5catdead-blast
Plasmid#220869Purposelentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequenceDepositorInsertbeta-1,3-galactosyltransferase 5 (B3GALT5 Human)
UseLentiviralTagsmyc, FLAGExpressionMammalianMutationencodes D156A and D243A mutations; also, nucleoti…PromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV:eGFP-p2A-HA-GPR151
Plasmid#190133Purposeexpresses eGFP-p2A-HA-GPR151 (wild-type variant) in mammalian cellsDepositorInserteGFP-p2A-HA-GPR151
UseLentiviralTagsHA, eGFP, and p2AExpressionMammalianMutationPromoterCMVAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV:eGFP-p2A-HA-GPR151(p.Arg95Ter)
Plasmid#190134Purposeexpresses eGFP-p2A-HA-GPR151 (pArg95Ter variant) in mammalian cellsDepositorInserteGFP-p2A-HA-GPR151(p.Arg95Ter)
UseLentiviralTagsHA, eGFP, and p2AExpressionMammalianMutationp.Arg95Ter mutationPromoterCMVAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mouse Ism1 with C-terminal Myc-6X-his tag
Plasmid#173046PurposeExpresses Ism1 in mammalian cellsDepositorInsertIsm1 (Ism1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
-
pET-21a(+)-LpxA
Plasmid#12684DepositorInsertUDP-N-acetylglucosamine acyltransferase (HP1375 Helicobacter pylori)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUCdeltaSS-26X
Plasmid#108218PurposeYeast reporter gene with glucocorticoid response element (GRE) upstream of CYC1 promoter driving lacZ expressionDepositorInsertGRE-CYC1-lacZ
UseTagsExpressionYeastMutationPromoterCYC1Available sinceSept. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pJKR-H-benM
Plasmid#73925PurposeMuconate biosensor. Expresses GFP proportional to muconate concentration within the cell.DepositorInsertmuconate biosensing apparatus
UseTagsExpressionMutationPromoterAvailable sinceJan. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
-
TRPML3-YFP
Plasmid#18835DepositorInsertTRPML3 (Mcoln3 Mouse)
UseTagsYFPExpressionMammalianMutationQ165R, F210YPromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only
