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Plasmid#186846PurposeDoxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transductionDepositorInsertOrangutan cGAS truncation mutant (158-521 a.a.) with C-terminal GFP tag (CGAS Pongo abelii, Synthetic)
UseRetroviralTagsGFPExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-chimpanzee cGASdeltaN-GFP
Plasmid#186847PurposeDoxycycline-dependent expression of chimpanzee cGAS gene in mammalian cells by retroviral transductionDepositorInsertChimpanzee cGAS truncation mutant (160-520 a.a.) with C-terminal GFP tag (CGAS Pan troglodytes, Synthetic)
UseRetroviralTagsGFPExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-FLAG-human cGASdeltaN-HA
Plasmid#186848PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Human, Synthetic)
UseRetroviralTagsFLAG and HAExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-FLAG-human cGAS-HA
Plasmid#186849PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS with N-terminal FLAG and C-terminal HA tag (Adamts1 Human)
UseRetroviralTagsFLAG and HAExpressionMutationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-EGFP-IRES(Neo)R
Plasmid#158068PurposeeGFP under the TRE3G promoter, Neo resistance. For cloning GOIDepositorInserteGFP
UseTagseGFPExpressionMammalianMutationPromoterTRE3GAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-IRSp53 P428L
Plasmid#221321PurposeDox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transductionDepositorInsertIRSp53 (Baiap2 Mouse)
UseRetroviralTags3xFLAGExpressionMutationP428LPromoterTRE3GAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-GMIP P564D
Plasmid#221324PurposeDox-inducible expression of GMIP mutant in mammalian cells by retroviral transductionDepositorInsertGMIP (Gmip Mouse)
UseRetroviralTags3xFLAGExpressionMutationP564DPromoterTRE3GAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-GMIP S31D
Plasmid#221322PurposeDox-inducible expression of GMIP mutant in mammalian cells by retroviral transductionDepositorInsertGMIP (Gmip Mouse)
UseRetroviralTags3xFLAGExpressionMutationS31DPromoterTRE3GAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-IRSp53 S269D
Plasmid#221319PurposeDox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transductionDepositorInsertIRSp53 (Baiap2 Mouse)
UseRetroviralTags3xFLAGExpressionMutationS269DPromoterTRE3GAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR-U6-gRNA-TRE3G-Nluc
Plasmid#192659PurposeA plasmid encoding TRE3G sgRNA and TRE3G-promoter-NlucDepositorInsertNluc
UseLuciferaseTagsExpressionMammalianMutationPromoterU6, TRE3GAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMVTRE3G_LYSET-Isoform2-I142V-Flag
Plasmid#192006PurposeLentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 I142V mutant stable expressing cell line under CMVTRE3G promoterDepositorInsertLYSET-Isoform2-I142V-Flag (LYSET Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVTRE3GAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMVTRE3G_LYSET-Isoform2-R45W-Flag
Plasmid#192005PurposeLentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 R45W mutant stable expressing cell line under CMVTRE3G promoterDepositorInsertLYSET-Isoform2-R45W (LYSET Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVTRE3GAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-NODALvar-matMYC
Plasmid#115641PurposeFor targeted integration and inducible expression of a human NODAL splice variant (with an N-terminal MYC tag on the mature peptide) using doxycyclineDepositorInsertNODAL splice variant (NODAL Human)
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPD1023 TRE3GV DsRedExpress2 in TU5
Plasmid#139257PurposeTRE3GV promoter and DsRedExpress2 in TUPV5DepositorInsertDsRedExpress2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD767 TRE3GV MCS in TU4
Plasmid#139256PurposeTRE3GV promoter and an MCS in TUPV4DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-PYL1-sfGFP-PML
Plasmid#167011Purposeexpression of PYL1-SfGFP-PML, lentivirus vectorDepositorInsertPML (PML Human)
UseLentiviralTagsGFP-PMLExpressionMammalianMutationPromoterAvailable sinceJune 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3G-p300-dCas9-P2A-mCherry
Plasmid#138456Purpose2nd Generation Lentiviral vector. Expresses an N-terminal p300-dCas9 fusion protein and mCherryDepositorInsertp300 core
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3G-LSD1-dCas9-P2A-mCherry
Plasmid#138462Purpose2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherryDepositorInsertLSD1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVTRE3G eGFP Blast (w818-1)
Plasmid#27568DepositorInsertEnhance Green Fluorescent Protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV[TetOn]-Puro-TRE3G>mAscl1
Plasmid#198755PurposeVector encodes the gene for murine Ascl1 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoterDepositorInsertAscl1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMutationPromoterTRE3GAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only