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Plasmid#118722PurposeThe donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry(Y72L)] (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherry(Y72L)ExpressionMammalianMutationtruncation after aa1353; Y72L mutation in mCherry…PromoterCMVAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I acceptor only control (F40-based tension sensor)
Plasmid#119189PurposeThe acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.DepositorInserthuman Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I donor only control (F40-based tension sensor)
Plasmid#119188PurposeThe donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.DepositorInserthuman Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II donor only control (F40-based tension sensor)
Plasmid#118717PurposeThe donor (YPet(short)) only control for the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II-[YPet(short)-F40-mCherry(Y72L)] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherry(Y72L)ExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II donor only control (F40-based no force control)
Plasmid#118718PurposeThe donor (YPet(short)) only control for the no force control of the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry(Y72L)] (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherry(Y72L)ExpressionMammalianMutationtruncation after aa1353; Y72L mutation in mCherry…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II acceptor only control (F40-based tension sensor)
Plasmid#118719PurposeThe acceptor (mCherry) only control for the F40-based human desmoplakin II tension sensor can be co-expressed with the donor (YPet(short)) only control to determine intermolecular FRET.DepositorInserthuman Desmoplakin II-[YPet(short)(Y67G)-F40-mCherry] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)(Y67G)-F40-mCherryExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II acceptor only control (F40-based no force control)
Plasmid#118720PurposeThe acceptor (mCherry) only control for the no force control of the F40-based human desmoplakin II tension sensor can be co-expressed with the donor only control to determine intermolecular FRET.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)(Y67G)-F40-mCherry] (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherry(Y72L)ExpressionMammalianMutationtruncation after aa1353; Y67G mutation in YPet(sh…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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GP803
Plasmid#164547PurposeExpresses Smo-FLAG K434, 444, 448RDepositorInsertSmoothened K434, 444, 448R (Smo Mouse)
UseLentiviralTagsFLAGExpressionMutationAll three Lysines in cytoplasmic loop three of Sm…PromoterGgCryD1Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GP802
Plasmid#164546PurposeExpresses Smo-FLAG K348, 360RDepositorInsertSmoothened K348, 360R (Smo Mouse)
UseLentiviralTagsFLAGExpressionMutationBoth the Lysines in cytoplasmic loop two of Smoo…PromoterGgCryD1Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GP826
Plasmid#164551PurposeExpresses Smo-FLAG K444, 448RDepositorInsertSmoothened K444, 448R (Smo Mouse)
UseLentiviralTagsFLAGExpressionMutationTwo of the three Lysines in loop three of Smoothe…PromoterGgCryD1Available sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
GP825
Plasmid#164550PurposeExpresses Smo-FLAG K434, 448RDepositorInsertSmoothened K434, 448R (Smo Mouse)
UseLentiviralTagsFLAGExpressionMutationTwo of the three Lysines in loop three of Smoothe…PromoterGgCryD1Available sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
GP824
Plasmid#164549PurposeExpresses Smo-FLAG K434, 444RDepositorInsertSmoothened K434, 444R (Smo Mouse)
UseLentiviralTagsFLAGExpressionMutationTwo of the three Lysines in loop three of Smoothe…PromoterGgCryD1Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only