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Plasmid#1138DepositorInsertsup35 delta 2-5 mutant, 5' and 3' flanking regions (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationlast four repeats of sup35 N domain deleted, but …PromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCA-ATG-intron-tdT3Myc
Plasmid#36883DepositorInserttdTomato-3Myc
UseTags3 Myc tagsExpressionMammalianMutationbeta-globin intron containing a loxP site inserte…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTK-enhNC1.1_M3-MCS-2A-eGFP-SV40pA
Plasmid#110203PurposeRecipient vector for Cranial Neural Crest-specific expression of protein of interestDepositorInsertenhNC1.1_M3-TF_MCS-2A-eGFP-SV40pA
UseTagseGFPExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash5
Plasmid#74133PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash6
Plasmid#74134PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash1
Plasmid#74129PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash4
Plasmid#74132PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash3
Plasmid#74131PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash2
Plasmid#74130PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 βarr1 S412D Flag
Plasmid#42196DepositorInsertβ-arrestin 1 S412D (Arrb1 Rat)
UseTagsFlagExpressionMammalianMutationS412DPromoterCMVAvailable sinceJan. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 βarr1 S412A Flag
Plasmid#42195DepositorInsertβ-arrestin 1 S412A (Arrb1 Rat)
UseTagsFlagExpressionMammalianMutationS412APromoterCMVAvailable sinceJan. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe.puro.CDK8.KD.flag
Plasmid#19759DepositorInsertcyclin-dependent kinase 8 (CDK8 Human)
UseRetroviralTagsFlagExpressionMammalianMutationD173A (kinase dead)PromoterAvailable sinceDec. 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBabe.puro.CDK8.flag
Plasmid#19758DepositorInsertcyclin-dependent kinase 8 (CDK8 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1.shCDK8
Plasmid#19760DepositorInsertsmall hairpin RNA against CDK8 (CDK8 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1.sh.beta-catenin.1248
Plasmid#19761DepositorInsertsmall hairpin RNA against beta-catenin (CTNNB1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1.sh.beta-catenin.2279
Plasmid#19762DepositorInsertsmall hairpin RNA against beta-catenin (CTNNB1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilB
Plasmid#162845PurposeExpression of PilB with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBF120*
Plasmid#162852PurposeExpression of PilB F120 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationF120AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBT125*
Plasmid#162853PurposeExpression of PilB T125 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationT125AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBN189*
Plasmid#162854PurposeExpression of PilB N189 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationN189AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only