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pSLIK-NFLAG-hFUS

Plasmid

#50487

Purpose

Produces lentivirus expressing human FUS with FLAG tag at its N-terminus by co-transfection with psPAX2 and pMD2G

Depositor

Zissimos Mourelatos

Article

Nakaya et al RNA. 2013 Apr;19(4):498-509. doi: 10.126

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

TRE

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only
416Gal-SV40NLS-FUS-YFP

Plasmid

#29595

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

NLS-FUS (FUS Human)

Use

Tags

SV40 NLS and YFP

Expression

Yeast

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-RRM Mutant-YFP

Plasmid

#29608

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS RRM mutant (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

F305L, F341L, F359L and F368L

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
RP1B FUS 1-163 QQ4xSS #2

Plasmid

#127195

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 QQ4xSS #2 (FUS Human)

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

QQ4xSS #2:Q8S, Q9S, Q60S, Q62S, Q102, Q103, Q139S…

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-FUSN-miRFP670-Cry2WT

Plasmid

#122441

Purpose

Expresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
426Gal-FUS-1-501aa-YFP

Plasmid

#29601

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-501aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-501

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-170aa-YFP

Plasmid

#29596

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-170aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-170

Promoter

Available since

April 29, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-373aa-YFP

Plasmid

#29598

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-373aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-373

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-413aa-YFP

Plasmid

#29599

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-413aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-413

Promoter

Available since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-101-526aa-YFP

Plasmid

#29603

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-101-526aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 101-526

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-453aa-YFP

Plasmid

#29600

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-453aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-453

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-267-526aa-YFP

Plasmid

#29605

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-267-526aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 267-526

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-285-526aa-YFP

Plasmid

#29606

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-285-526aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 285-526

Promoter

Available since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-270aa-YFP

Plasmid

#29597

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-270aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-270

Promoter

Available since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-368-526aa-YFP

Plasmid

#29607

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-368-526aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 368-526

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
M23: pHR-SFFVp-FUSN::mCherry::SspB

Plasmid

#122148

Purpose

N terminal domain of FUS (1-214) tagged with SspB and mCherry.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

mCherry-sspB

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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3X FLAG-FUS-WT

Plasmid

#44985

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (Full length) (FUS Human)

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
TH1204-pOCC120-FUS_opt(NheI)

Plasmid

#221892

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Use

Tags

MBP-PreScission and mGFP-PreScission-6xHis

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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GST-FUS-WT

Plasmid

#44978

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (Full length) (FUS Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pVC-FUS-R521C

Plasmid

#175067

Purpose

expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells

Depositor

Arnold Boersma

Article

Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

Insert

FUS glycine rich protein (FUS Human)

Use

Tags

HA and mVenus + mCherry

Expression

Mammalian

Mutation

changed Arginin 521 to Cystein; mVenus: C-Termina…

Promoter

CMV Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge