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Plasmid#227248PurposeLambda DNA for torsional templatesDepositorInsertLambda DNA
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP33
Plasmid#212866PurposeExpresses the Streptomyces platensis LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationPromoterPcymRCAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP34
Plasmid#212867PurposeExpresses the Streptomyces platensis LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationPromoterPcymRCAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMH020
Plasmid#186633Purposecontains homology arms for the pilA (NIES39_C03030) locus of Arthrospira platensisDepositorArticleTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH021
Plasmid#186634Purposecontains homology arms for the NSI (NIES39_Q01230) locus of Arthrospira platensisDepositorArticleTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH022
Plasmid#186635Purposecontains homology arms for the oscillin (NIES39_A01430) locus of Arthrospira platensisDepositorArticleTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
SJZ7-KAN
Plasmid#228260PurposeTemplate plasmid for yeast gene knockout, confers antibiotic resistance to G418DepositorInsertkan
UseTagsExpressionYeastMutationPromoterGPM1 S.c.Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
SJZ4-NAT
Plasmid#228257PurposeTemplate plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricinDepositorInsertnat1
UseTagsExpressionYeastMutationPromoterFBA1 S.c.Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SJZ5-HPH
Plasmid#228258PurposeTemplate plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin BDepositorInserthph
UseTagsExpressionYeastMutationPromoterSSA1 S.c.Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
SJZ2-TRP1
Plasmid#228256PurposeTemplate plasmid for yeast gene knockout, uses auxotrophic marker TRP1 (Kluyveromyces lactis)DepositorInsertTRP1
UseTagsExpressionYeastMutationPromoterBBC1 S.c.Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SJZ9-amdS
Plasmid#228261PurposeTemplate plasmid for yeast gene knockout, allows the use of acetamide as sole nitrogen sourceDepositorInsertamdS
UseTagsExpressionYeastMutationPromoterENO2 S.c.Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SJZ10-LEU2
Plasmid#228262PurposeTemplate plasmid for yeast gene knockout, uses auxotrophic marker LEU2 (Kluyveromyces lactis)DepositorInsertLEU2
UseTagsExpressionYeastMutationPromoterLEU2 K.l.Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJYDN3
Plasmid#162456PurposeYeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pES133
Plasmid#127552PurposeVariant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13DepositorInsertF5-flanked tetracycline resistance cassette
UseTagsExpressionBacterialMutationMultiple SNPs in tetA (originally from pBR322) to…PromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
SJZ6-HIS5
Plasmid#228259PurposeTemplate plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)DepositorInserthis5
UseTagsExpressionYeastMutationPromoterTEF1 S.c.Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI152-pLenti-SiT-Cas12a
Plasmid#128405PurposeExpress both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI
Plasmid#116954PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP2 MCR-1
Plasmid#118404PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.DepositorInsertMCR-1
UseTagsExpressionBacterialMutationPromoterpLacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1
Plasmid#116963PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDD379
Plasmid#91834PurposeSapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only