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pX459-SpCas9-NG

Plasmid

#171370

Purpose

SpCas9-NG-mediated genome editing.

Depositor

Masahito Ikawa

Article

Oura et al Commun Biol. 2021 Jun 23;4(1):771. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 for sgRNA; CBh for Cas9-2A-puroR

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Pou5f1

Plasmid

#174872

Purpose

CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO5d.EFS.SpCas9.P2A.PAC

Plasmid

#58329

Purpose

Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pX165-eSpCas9

Plasmid

#140564

Purpose

Expresses eSpCas9 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Use

Tags

Expression

Mutation

K848A K1003A R1060A

Promoter

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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SpCas9-mSA*

Plasmid

#182952

Purpose

Express SpCas9-mSA*

Depositor

Wen Xue

Article

Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

Insert

SpCas9-mSA*

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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P628_SpCas9-RecA

Plasmid

#87263

Purpose

Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

Depositor

Yonglun Luo

Article

Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

Insert

RecA

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

no

Promoter

CBh

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEC1.2/EC1.1_spCas9_TagRFP

Plasmid

#161934

Purpose

CRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selection

Depositor

Christian Fankhauser

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEc

Plasmid

#193834

Purpose

Express TadCBEc (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDc-SpCas9 D10A-2xUGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGGB-SpCas9

Plasmid

#210533

Purpose

Cloning A.thaliana codon optimized SpCas9 CDS as GreenGate module B

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

SpCas9

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-eSpCAS9-NG-pA

Plasmid

#131466

Purpose

Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

Insert

Codon optimized eSpCas9-NG

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g7

Plasmid

#80790

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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eSpCas9-plus

Plasmid

#126767

Purpose

Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

eSpCas9-plus

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

K848A, R1060A; amino acids 1005-1013 replaced wit…

Promoter

CBh

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

Plasmid

#172225

Purpose

Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Insert

sgRNA

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-SpCAS9-NG-pA

Plasmid

#131465

Purpose

Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

Insert

Codon optimized SpCas9-NG

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn spCas9

Plasmid

#179725

Purpose

human synapsin (hSyn)-driven spCas9 lentiviral vector for CRISPR/HITI

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

spCas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g9

Plasmid

#80792

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g8

Plasmid

#80791

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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SpCas9 iFE

Plasmid

#190138

Purpose

Expresses SpCas9-POLB (iFE)

Depositor

Timothy Lu

Article

Nakade et al bioRxiv 2022.12.05.518807

Insert

SpCas9-POLB (HiFi iFE)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Codon Optimized for mammalian cells

Promoter

CBh

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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p276 eSpCas9_2gRNAs_hH11

Plasmid

#164850

Purpose

gRNA vector for targeting human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

gRNAs for targeting human H11 locus

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

Plasmid

#110300

Purpose

Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

Depositor

Chris Kok-Lung Chan

Article

Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

Insert

p53-binding protein 1 (TP53BP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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