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pRSET-His-APEX2

Plasmid

#201471

Purpose

Recombinant soluble APEX2 for SLAPSHOT labeling

Depositor

Yuh-Nung Jan

Article

Tuomivaara et al Commun Biol. 2024 Aug 29;7(1):1060. doi:

Insert

APEX2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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SuperNova Green/ pRSETB

Plasmid

#124839

Purpose

bacterial expression of SuperNovaGreen, a green monomeric photosensitizing fluorescent protein

Depositor

Takeharu Nagai

Article

Riani et al BMC Biol. 2018 Apr 30;16(1):50. doi: 10.

Insert

SuperNova Green

Use

Tags

No tag

Expression

Bacterial

Mutation

Promoter

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE-600n

Plasmid

#13537

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET his-eGFP

Plasmid

#113551

Purpose

For bacterial expression and purification of eGFP

Depositor

Jeanne Stachowiak

Article

Busch et al Nat Commun. 2015 Jul 24;6:7875. doi: 10.

Insert

his-eGFP

Use

Tags

Expression

Bacterial

Mutation

A206K

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET iGluSnFR3 v82

Plasmid

#175187

Purpose

Fluorescent reporter for glutamate, third generation, variant 82 soluble form to express in bacteria.

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

iGluSnFR3 v82

Use

Synthetic Biology

Tags

His tag, T7 leader, Xpress epitope

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPPi-30m

Plasmid

#13556

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi T22A

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

T22A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET-WHaloCaMP1a-EGFP

Plasmid

#205303

Purpose

Expression of WHaloCaMP1a-EGFP fusion in E. coli

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET.GltI253-cpGFP.L1LV/L2NP

Plasmid

#41733

Purpose

a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Insert

GltI based Glutamate Biosensor

Use

Glutamate biosensor

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Bacterial

Mutation

cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

Promoter

T7

Available since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPPi-200u

Plasmid

#13555

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi G162A

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

G162A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pRsetB-his7-Perceval

Plasmid

#20336

Depositor

Gary Yellen

Article

Berg et al Nat Methods. 2009 Feb . 6(2):161-6.

Insert

Perceval (fluorescent ATP:ADP sensor)

Use

Tags

his(7)

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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Nano-lantern/pRSETB

Plasmid

#51969

Purpose

bacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pRSET mNG-GECO1

Plasmid

#201933

Purpose

Expresses mNG-GECO1 indicator in bacteria

Depositor

Robert Campbell

Article

Zarowny et al ACS Sens. 2020 Jul 24;5(7):1959-1968. do

Insert

mNeonGreenGECO1

Use

Tags

HIS tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSETb-Ferritin-Dronpa

Plasmid

#101194

Purpose

Expresses a Ferritin-Dronpa fusion construct in E. coli

Depositor

Peter Dedecker

Article

Duwe et al Chem Commun (Camb). 2017 Jun 29;53(53):7

Insert

Ferritin-Dronpa

Use

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET FLII12Pglu-700uDelta6

Plasmid

#13568

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLIPglu F16A Y12S-ECFP-D13R, 6aa linker

Use

Tags

Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in mglB

Promoter

Available since

Feb. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE intramol-1u

Plasmid

#13549

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol WT (gltI E. coli)

Use

Tags

EYFP

Expression

Bacterial

Mutation

ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSETb-tVYN-TmΔ

Plasmid

#149694

Purpose

High-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purification

Depositor

Ming Hammond

Article

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-TmYcgRΔ-NLucΔ4

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETB PKA Cat

Plasmid

#14920

Depositor

Susan Taylor

Article

Taylor et al Biochim Biophys Acta. 2005 Dec 30. 1754(

Insert

PKA catalytic subunit alpha (Prkaca Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2007

Availability

Academic Institutions and Nonprofits only
pRSET FLIPglu-600uDelta13

Plasmid

#13559

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A Delta13

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET-WHaloCaMP1b-EGFP

Plasmid

#205305

Purpose

Expression of WHaloCaMP1b-EGFP fusion in E. coli

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1b-EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET FLIPPi-260n

Plasmid

#13552

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi WT

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge