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pFastBac-His-Flag-Timeless

Plasmid

#22892

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Timeless (TIMELESS Human)

Use

Tags

Flag and His

Expression

Insect

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-Tag2B Flag BimEL(TA)

Plasmid

#23093

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (TA) (Bcl2l11 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed Threonine 112 to Alanine

Promoter

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-Tag2B Flag BimEL(TD)

Plasmid

#23092

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (TD) (Bcl2l11 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed Threonine 112 to Aspartate

Promoter

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pBEST-melR-deGFP-T500

Plasmid

#45396

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

melibiose repressor

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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Venus-BimEL(Noxa)-pEGFP-C1

Plasmid

#166761

Purpose

To examine effect of swapping the BH3 region in full length BimEL with that of Noxa

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

BimEL(Mus musculus)-(Noxa BH3) (Bcl2l11, PMAIP1 Mouse, Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

BH3 region removed and inserted BH3 of human Noxa…

Promoter

CMV

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pMP71-mGFP-LLO-hPMEL

Plasmid

#174607

Purpose

expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the human hPMEL epitope

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

expresses palmitoylated GFP w/C-term fusion of the LLO190 epitope and human hPMEL epitope

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-BimEL(Bik)-pEGFP-C1

Plasmid

#166760

Purpose

To examine effect of swapping the BH3 region in full length BimEL with that of Bik

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

BimEL(Mus musculus)-(Bik BH3) (Bcl2l11, BIK Mouse, Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

BH3 region removed and inserted BH3 of human Bik …

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-BimEL(Bid)-pEGFP-C1

Plasmid

#166759

Purpose

To examine effect of swapping the BH3 region in full length BimEL with that of Bid

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

BimEL(Bid) (Bcl2l11, Bid Mouse)

Use

Tags

Venus

Expression

Mammalian

Mutation

BH3 region removed and inserted BH3 of mouse Bid …

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-BimEL(Bad)-pEGFP-C1

Plasmid

#166758

Purpose

To examine effect of swapping the BH3 region in full length BimEL with that of Bad

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

BimEL(Mus musculus)-(Bad BH3) (Bcl2l11, BAD Mouse, Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

BH3 region removed and inserted BH3 of human Bad …

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-Venus-FLARE-Cameleon

Plasmid

#123351

Purpose

Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-Venus-FLARE-Cameleon

Use

Tags

6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon

Plasmid

#123353

Purpose

Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-Cerulean3-FLARE-Cameleon

Use

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-mCherry-FLARE-Cameleon

Plasmid

#123352

Purpose

Red-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

mCherry-mCherry-FLARE-Cameleon

Use

Tags

6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-Cameleon

Plasmid

#123350

Purpose

Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-Cameleon

Use

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-TetOn-Puro_MeltITSN1-37-mch

Plasmid

#234068

Purpose

Encodes the Dbl homology and pleckstrin homology (DH-PH) domain of Intersectin1 controlled by a Melt variant with a switching temperature of 37°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltITSN1-37-mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA)

Plasmid

#160369

Purpose

Stable expression of GPR143-mScarlet-3xHA fusion protein for the isolation of melanosomes.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

GPR143 (GPR143 Human)

Use

Lentiviral

Tags

mScarlet-3xHA

Expression

Mutation

Promoter

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Drosophila melanogaster CBS-pair domain

Plasmid

#166436

Purpose

Express pGEX-6P-1 Drosophila melanogaster CBS-pair domain

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Drosophila melanogaster CBS-pair domain (Cbs Fly)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Tac, LacI, Amp

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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MelanoTag-Control-PURO (pLJC5-GPR143-mScarlet-1xMyc)

Plasmid

#160368

Purpose

Stable expression of GPR143-mScarlet-1xMyc fusion protein for use as a control with MelanoTag-PURO

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

GPR143 (GPR143 Human)

Use

Lentiviral

Tags

mScarlet-myc

Expression

Mutation

Promoter

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)

Plasmid

#166418

Purpose

Express pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Drosophila melanogaster PRL (Prl Fly)

Use

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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MTNR1A-DuET

Plasmid

#213348

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

MTNR1A (MTNR1A Human)

Use

Tags

1D4 and FLAG

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-RNF110

Plasmid

#126547

Purpose

Expresses GFP-tagged RNF110 in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF110 (PCGF2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

LTR

Available since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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