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pECE M2-SH3PXD2A 8 serines mutated to alanines

Plasmid

#69816

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-Fascin-Promoter (-402-0)

Plasmid

#89823

Purpose

Promoter luciferase assay

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Fascin1 (FSCN1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

SV40

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pECE M2-SH3PXD2A 6 serines mutated to alanines

Plasmid

#69815

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST NET1A S46A

Plasmid

#69818

Purpose

Expresses NET1A-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

S46A

Promoter

CMV

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-Fascin-Promoter (-210-0)

Plasmid

#89826

Purpose

Fascin promoter for luciferase assay

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Fascin1 (FSCN1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

SV40

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST CDC42EP1 WT

Plasmid

#69819

Purpose

Expresses CDC42EP1-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

CDC42 effector protein (CDC42EP1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-Fascin-Promoter 402 mutation

Plasmid

#89825

Purpose

Fascin promoter for luciferase assay

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Fascin1 (FSCN1 Human)

Use

Luciferase

Tags

Expression

Mutation

-370 Smad binding element mutated from CAGAC to T…

Promoter

SV40

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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FUW TetO NET1A-V5 S46A

Plasmid

#69829

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

S46A

Promoter

minimal CMV promoter with tetracycline operator

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST CDC42EP1 S192A

Plasmid

#69820

Purpose

Expresses CDC42EP1-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

CDC42 effector protein (CDC42EP1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

S192A

Promoter

CMV

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-SOUL-P2A-tdTomato

Plasmid

#153019

Purpose

Step-function opsin with ultra-high light sensitivity (SOUL). Capable to be used for noninvasive stimulation in mice and minimally invasive stimulation in macaque.

Depositor

Guoping Feng

Article

Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

Insert

SOUL

Use

AAV

Tags

P2A-tdTomato

Expression

Mammalian

Mutation

C128S/D156A/T159C

Promoter

Available since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO EYFP

Plasmid

#27056

Depositor

Karl Deisseroth

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

Insert

EYFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pFIP

Plasmid

#17565

Depositor

Jay Keasling

Article

Ham et al Biotechnol Bioeng. 2006 May 5. 94(1):1-4

Insert

inversion switch

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pT2/SVNeo

Plasmid

#26553

Depositor

Perry Hackett

Article

Cui et al J Mol Biol. 2002 May 17. 318(5):1221-35.

Insert

pT2/SVNeo

Use

Tags

Expression

Bacterial

Mutation

added the Rmut(80, 87, d101)

Promoter

Available since

March 14, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3 luciferase

Plasmid

#26612

Depositor

Jie Chen

Article

Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

Insert

firefly luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3 RRM2

Plasmid

#13796

Depositor

Edward Whang

Article

Duxbury et al Biochem Biophys Res Commun. 2007 Mar 2.

Insert

RRM2 (RRM2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2007

Availability

Academic Institutions and Nonprofits only

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Flag-SRm160

Plasmid

#11305

Depositor

Phil Sharp

Article

Cheng et al Mol Cell Biol. 2006 Jan . 26(1):362-70.

Insert

SRm160 (SRRM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pDest490-OPN-a

Plasmid

#17590

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-a (SPP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 49–942

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-TOP luciferase

Plasmid

#26611

Depositor

Jie Chen

Article

Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

Insert

eEF2 5'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-Ii31

Plasmid

#64598

Purpose

Expression of invariant chain as model antigen for study of MHC class II presentation

Depositor

Martin Zenke, Sandra Diebold

Article

Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Insert

MHC II Invariant Chain

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pDest490-OPN-c

Plasmid

#17592

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-c (SPP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 1–93, 175–942

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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