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Plasmid#44211DepositorInsertCBF1-Responsive-Element
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pH2B-PSmOrange
Plasmid#31920DepositorInsertPSmOrange
UseTagsH2BExpressionMammalianMutationS21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…PromoterAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pH2B-LSSmOrange
Plasmid#37133DepositorInsertLSSmOrange
UseTagshistone 2BExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable sinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pH2B-LSSmKate2-N1
Plasmid#31903DepositorInsertH2B-LSSmKate2
UseTagsExpressionMammalianMutationK69Y/P131T/S148G/M167D/T183S/M196V compared to mK…PromoterCMVAvailable sinceSept. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBS-PtH2B
Plasmid#133390PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterNo promoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-C3H2B
Plasmid#133387PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti.tie1.H2B.eYFP.W
Plasmid#51293PurposeLentivector that encodes H2B-eYFP under the transcriptional control of the mouse TIE1 promoterDepositorInsertH2B-eYFP (H2BC21 Human)
UseLentiviralTagsH2B-eYFPExpressionMutationPromotermTie1Available sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPB: PGK-H2B-mIFP-T2A-rTetR-GBP-polyA-zeo
Plasmid#163084PurposerTetR DNA binding domain fused to GBP (anti-GFP nanobody)DepositorInsertanti-GFP nanobody
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-K122-TEAD-p-H2B-mCherry
Plasmid#139648PurposeReporter assayDepositorInsertIRES-GFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry
Plasmid#159655PurposeEmpty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH2B-6AA-ffDronpa
Plasmid#214606PurposeExpresses reversibly switchable fluorescent protein ffDronpa targeted to the nucleusDepositorArticleInsertffDronpa-H2B
UseTagsH2B targetting with a linker of 6AAExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
XRE-H2B-mScarlet-I
Plasmid#182296PurposeFluorescent reporter for AhR activityDepositorInsertmScarlet-I
UseTagsm-Scarlet-IExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry
Plasmid#139649PurposeReporter assayDepositorInsertH2B-mCherry
UseLentiviralTagsFLAG, His6ExpressionMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 H2B-mIFP T2A Mpro (wt)
Plasmid#163079PurposeExpresses wild type Mpro (active) and a near-infrared fluorescent protein mIFP fused to H2BDepositorInsertWild type Mpro of SARS-CoV-2 and H2B-mIFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-squirrel-monkey-retroCHMP3-T2A-H2B-BFP
Plasmid#154185Purposeexpresses squirrel monkey retroCHMP3 with self-cleaving tag in mammalian cellsDepositorInsertretroCHMP3
UseTagsHA and T2A-H2B-BFPExpressionMammalianMutationPromoterEF1-alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 H2B-mIFP T2A Mpro (C145A)
Plasmid#163080PurposeExpresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2BDepositorInsertInactive Mpro mutant C145A and H2B-mIFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Neo-CAG-M2rtTA-H2BmNeon (donorAB-mNeon)
Plasmid#85799PurposedonorAB-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFPDepositorInsertPPP1R12C (PPP1R12C Human)
UseHuman targetingTagsExpressionMutationhomology arms for knock-in into first intronPromoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-nucGFP11-3xControlgRNA
Plasmid#224568PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)ExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nucGFP11-3xPax7gRNA
Plasmid#224570PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)ExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only