Skip to main content
Addgene
Showing: 121 - 140 of 4743 results

  2. pCRISPR-cBEST

    Plasmid
    #125689
    Purpose
    C to T base editor for actinomycetes
    Depositor
    Tilmann Weber
    Article
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Insert
    streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    ermE*/tipA
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    125689-plasmid-map-sequence-id-271202

    Enlarge

  3. pCRISPR-aBEST

    Plasmid
    #131464
    Purpose
    Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes
    Depositor
    Tilmann Weber
    Article
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Inserts
    Streptomyces codon optimized spCas9n (D10A)
    streptomyces codon optimized tadA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    tipA
    Available since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    131464-plasmid-map-sequence-id-261686

    Enlarge

  5. pBAD-bHs

    Plasmid
    #134259
    Purpose
    For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag
    Depositor
    Edwin Manuel
    Article
    Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7
    Insert
    Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    codon optimized for Salmonella typhimurium
    Promoter
    AraC
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    134259-plasmid-map-sequence-id-264461

    Enlarge

  6. pmCellFree_KA1_SARS-COV-2_ORF7a

    Plasmid
    #169393
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7a with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF7a (ORF7a SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169393-plasmid-map-sequence-id-336506

    Enlarge

  7. pLSU1/t35s[TMV]:mNeonGreen-I{PDK-198}:NosT

    Plasmid
    #212170
    Purpose
    This binary vector expresses mNeonGreen containing the castor bean catalase (PDK) intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTR
    Depositor
    Wayne Curtis
    Insert
    Tomato codon optimized version of mNeonGreen-I{PDK-198}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PDK int…
    Promoter
    truncated 35sCaMV
    Available since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212170-plasmid-map-sequence-id-422699

    Enlarge

  8. pLSU1/t35s[TMV]:mNeonGreen-I{AGO-198}:NosT

    Plasmid
    #212169
    Purpose
    This binary vector expresses mNeonGreen containing the Argonaut (AGO) intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTR
    Depositor
    Wayne Curtis
    Insert
    Tomato codon optimized version of mNeonGreen-I{AGO-198}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and AGO int…
    Promoter
    truncated 35sCaMV
    Available since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212169-plasmid-map-sequence-id-422695

    Enlarge

  9. pBHM2403

    Plasmid
    #171687
    Purpose
    pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.
    Depositor
    Hiten Madhani
    Article
    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi
    Insert
    CnoCAS9
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    TEF1
    Available since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    171687-plasmid-map-sequence-id-340174

    Enlarge

  12. pDmelOR-mRho.V5.mER.hOr56a

    Plasmid
    #126479
    Purpose
    Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Inserts
    Or56a (Or56a Fly)
    Orco (Orco Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens and codon optim…
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126479-plasmid-map-sequence-id-246521

    Enlarge

  14. pAaePUb::Cas9-2A-Neo

    Plasmid
    #176680
    Purpose
    Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)
    Depositor
    Norbert Perrimon
    Article
    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:
    Insert
    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    Use
    Crispr
    Tags
    Expression
    Insect
    Mutation
    Drosophila codon optimized
    Promoter
    Ae. aegypti poly-ubiquitin (AAEL003888)
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    176680-plasmid-map-sequence-id-348671

    Enlarge

  16. pTEF:ATP

    Plasmid
    #92179
    Purpose
    HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.
    Depositor
    Matthias Heinemann
    Article
    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi
    Insert
    ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
    Use
    Integration and expression into yeast.
    Tags
    Yeast codon optimized mseCFP (donor) and cp173-mV…
    Expression
    Yeast
    Mutation
    Promoter
    TEF1p
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92179-plasmid-map-sequence-id-181610

    Enlarge

  17. pLSU1/t35s[]:mNeonGreen-CI{PIV-198}:NosT

    Plasmid
    #212171
    Purpose
    This binary vector expresses mNeonGreen containing the PIV intron (198bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter
    Depositor
    Wayne Curtis
    Insert
    Tomato codon optimized version of mNeonGreen-I{PIV-198}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PIV int…
    Promoter
    truncated 35sCaMV
    Available since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212171-plasmid-map-sequence-id-422717

    Enlarge

Showing: 121 - 140 of 4743 results