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pZR047_Lenti-U6-sgGARP-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

Plasmid

#180268

Purpose

Lentiviral expression vector for CRISPRa-SAM with GARP targeting sgRNA

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

GARP sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG MCP-VP64

Plasmid

#92357

Purpose

CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

MCP-VP64

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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Raichu-OsRac1 CA

Plasmid

#133264

Purpose

Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

Depositor

Yoji Kawano

Article

Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

Insert

OsRac1(G19V) (LOC4325879 Oryza sativa)

Use

Tags

CFP-lipid and Venus-CRIB

Expression

Plant

Mutation

OsRac1 G19V

Promoter

Ubi

Available since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-TAZ-N-Flag

Plasmid

#19025

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Vector has no SV40 origin

Promoter

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pEF-TAZ-N-Flag (S89A)

Plasmid

#19026

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Vector has no SV40 origin, S89A (site directed mu…

Promoter

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-mTAZ-WW (86)

Plasmid

#40915

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Contains mTAZ 14-3-3 binding site and WW domain (…

Promoter

Tac

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-mTAZ-WW (43)

Plasmid

#40916

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Contains mTAZ WW domain (43 amino acids, 122-164)

Promoter

Tac

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pEF-TAZ-N-Flag (-4)

Plasmid

#40914

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Vector has no SV40 origin, and lacks the final fo…

Promoter

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
A3RXB7

Plasmid

#177694

Purpose

expresses a N-acetyl hexosamine oxidase in E. coli

Depositor

Martin Engqvist

Article

Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

Insert

N-acetylhexosamine oxidase

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Raichu-OsRac1 WT

Plasmid

#133262

Purpose

Biosensor for monitoring activation for rice small GTPase OsRac1

Depositor

Yoji Kawano

Article

Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

Insert

OsRac1 (LOC4325879 Oryza sativa)

Use

Tags

CFP-lipid and Venus-CRIB

Expression

Plant

Mutation

Promoter

Ubi

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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RSV-Pit1

Plasmid

#24059

Depositor

Herbert Samuels, Frederick Stanley

Article

Fox et al Mol Endocrinol. 1990 Jul . 4(7):1069-80.

Insert

Pit1 (SLC20A1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
Raichu-OsRac1 DN

Plasmid

#133263

Purpose

Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

Depositor

Yoji Kawano

Article

Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

Insert

OsRac1(T24N) (LOC4325879 Oryza sativa)

Use

Tags

CFP-lipid and Venus-CRIB

Expression

Plant

Mutation

OsRac1 T24N

Promoter

Ubi

Available since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ABKAR(T/A)

Plasmid

#140052

Purpose

Negative control mutant for ABKAR biosensor.

Depositor

Jin Zhang

Article

Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

Insert

ABKAR(T/A)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available since

Nov. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101102

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

TMt-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

HA and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101107

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_RFP

Plasmid

#206177

Purpose

mammalian expression of RFP

Depositor

Christopher Goodnow

Article

Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

Insert

RFP

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-Flag-TRAF5

Plasmid

#102679

Purpose

retroviral expression plasmid for TRAF5

Depositor

Hasam Habelhah

Article

Zhang et al J Mol Biol. 2009 Jun 12;389(3):495-510.

Insert

TRAF5 (Traf5 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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Scarlet I-FRET 10-NeonGreen

Plasmid

#139364

Purpose

FRET Standard

Depositor

Richard Day

Article

Reissaus et al Biophys J. 2020 Apr 21;118(8):1820-1829.

Insert

Scarlet 10AA Neon green

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pdCas9

Plasmid

#46569

Purpose

Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

Depositor

Luciano Marraffini

Article

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
dcas9
CRISPR array

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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