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Plasmid#169732PurposeEqualizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.DepositorInsertmiR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
3XFLAG_ALDOA in pCW57_tTA_Blast
Plasmid#154210PurposeDox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of ALDOADepositorInsertALDOA (ALDOA Human)
UseLentiviralTags3X FLAGExpressionMammalianMutationPromoterTRE repeats (dox off)Available sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRi(v1)-Blast
Plasmid#170067PurposeExpresses dCas9 repressor KRAB-dCas9 and blasticidin resistanceDepositorInsertKRAB-dCas9
UseCRISPR and LentiviralTags2A and 2xHA tagExpressionMammalianMutationPromoterEFS-NSAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
qTAG-KO-Blast
Plasmid#227263PurposeEmpty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Blast cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TPI in pCW57_tTA_Blast
Plasmid#154209PurposeDox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPIDepositorInsertTPI1 (TPI1 Human)
UseLentiviralTagsNoneExpressionMammalianMutationPromoterTRE repeats (dox off)Available sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-subtilase A
Plasmid#35948DepositorInsertSubtilase A (subA E. Coli)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLux76-LasI
Plasmid#73444PurposeHSL relay device: produces 3O-C12-HSL in response to 3O-C6-HSLDepositorInsertLasI
UseSynthetic BiologyTagsExpressionMutationPromoterpLux76Available sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Accessory plasmid 1
Plasmid#128569Purposefor mixing with SPECS library to produce lentivirusDepositorInsertECFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV+ plasmid
Plasmid#169740PurposeUnregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLas81-LuxI
Plasmid#73445PurposeHSL relay device: produces 3O-C6-HSL in response to 3O-C12-HSLDepositorInsertLuxI
UseSynthetic BiologyTagsExpressionMutationPromoterpLas81Available sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
mc2 blast
Plasmid#206217PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
Accessory plasmid 2
Plasmid#128571Purposefor cloning identified individual SPECSDepositorTypeEmpty backboneUseLentiviralTagsTagRFPExpressionMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash6
Plasmid#74134PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWZL Blast ST
Plasmid#13805DepositorInsertSV40 small T antigen (SV40gp7 simian virus)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-V5
Plasmid#207686PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-V5 cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV-Blast_hCOL4A5-HA
Plasmid#198103Purposelentiviral expression of hCOL4A5 in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseLentiviralTagsHAExpressionMutationPromoterEF1aAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash1
Plasmid#74129PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfuNca-Plastid
Plasmid#190818PurposePlastid targeting GFP with ble resistant gene for zeocinDepositorInsertsDiatom plastid targeted GFP
Bleomycin resistance gene
UseTagsExpressionBacterialMutationPromoterCylindrotheca fusiformis fcp and Nitzschia captiv…Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash4
Plasmid#74132PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash3
Plasmid#74131PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only