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pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG

Plasmid

#86871

Purpose

To express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

SF3B6 (SF3B6 Human)

Use

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

amino acids 7-29 deletion

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_SEPT9_i1-14-GFP10_GFP11-14-SEPT7 Gmut2

Plasmid

#180359

Purpose

mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN 9 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

bidirectional TRE

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_SEPT9_i3-14-GFP10_GFP11-14-SEPT7 Gmut2

Plasmid

#180361

Purpose

mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

bidirectional TRE

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Rock1-GBD (840-110 aa) in pEGFPN1

Plasmid

#187279

Purpose

Express EGFP-Rock1 GTPase-binding domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

GBD (84-110aa)

Promoter

CMV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)

Plasmid

#165029

Purpose

Express cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-vsv-Mis12-dCEN-Incenp-EGFP

Plasmid

#108478

Purpose

expression of vsv-Mis12-dCEN-INCENP-EGFP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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human EB1 in pEGFP N1 to express EB1-GFP (JB131)

Plasmid

#39299

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA

Plasmid

#74513

Purpose

AAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.

Depositor

Guoping Feng

Article

Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

Insert

EGFP-p2A-EGFP-f

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_SEPT2 NCmut-14-GFP11_SEPT2 NCmut-14-GFP10

Plasmid

#180357

Purpose

mammalian co-expression of human SEPT2 NCmut fused to GFP10 and of human SEPT2 NCmut fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN2 (SEPTIN2 Human)
SEPTIN 2 (SEPTIN2 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT2 F20D, V27D

Promoter

bidirectional TRE

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_SEPT9_i1 NCmut-14-GFP11_SEPT9_i1 NCmut-14-GFP10

Plasmid

#180358

Purpose

mammalian co-expression of human SEPT9_i1 NCmut fused to GFP10 and of human SEPT9_i1 NCmut fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN9_v1 (SEPTIN9 Human)
SEPTIN9_v1 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT9_i1 I281D, M288D

Promoter

bidirectional TRE

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET GFP domain 11 LIC cloning vector (GFP C-11)

Plasmid

#30183

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Biotin-H6-TEV recognition and GFP domain 11

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_GFP10-14-SEPT7 Gmut2_GFP11-14-SEPT7 Gmut2

Plasmid

#180353

Purpose

mammalian co-expression of human SEPT7 Gmut2 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN7 (SEPTIN7 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

bidirectional TRE

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_GFP10-14-SEPT7 Gmut1

Plasmid

#180354

Purpose

mammalian co-expression of human SEPT7 Gmut1 fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN7 (SEPTIN7 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 W269A, H279D

Promoter

bidirectional TRE

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_SEPT9_i1 Gmut-14-GFP10

Plasmid

#180355

Purpose

mammalian co-expression of human SEPT9_i1 Gmut fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN 9 (SEPTIN9 )
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 W269A, H279D and SEPT9_i1 W520A, H530D

Promoter

bidirectional TRE

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_SEPT9_i3 Gmut-14-GFP10

Plasmid

#180356

Purpose

mammalian co-expression of human SEPT9_i3 Gmut fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN 9 (SEPTIN9 )
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 W269A, H279D and SEPT9_i3 W502A, H512D

Promoter

bidirectional TRE

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP TRE BI_SEPT9_i3 NCmut-14-GFP11_SEPT9_i3 NCmut-14-GFP10

Plasmid

#180360

Purpose

mammalian co-expression of human SEPT9_i3 NCmut fused to GFP10 and of human SEPT9_i3 NCmut fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN9_v3 (SEPTIN9 Human)
SEPTIN9_v3 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT9_i3 I263D, M270D

Promoter

bidirectional TRE

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET His6 GFP TEV LIC cloning vector (1GFP)

Plasmid

#29663

Depositor

Scott Gradia

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

His6-GFP-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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PCDNA3-PAR1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

Plasmid

#113608

Purpose

PPI reporter

Depositor

Xiaokun Shu

Article

Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

Insert

PAR1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET MBP GFP LIC cloning vector (MBP-msfGFP)

Plasmid

#29750

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Biotin - His6 - MBP -TEV and GFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)

Plasmid

#29725

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Biotin - His6 - TEV and GFP

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge