Addgene

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p{3P3-mTurquoise::FRT-RC,attB}

Plasmid

#182181

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

mTurquoise

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRB35

Plasmid

#72866

Purpose

template base vector for N-terminal tagging, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

base vector for N-terminal tags, Puro

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pRB36

Plasmid

#72861

Purpose

template base vector for N-terminal tagging, constitutive Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

base vector for introduction of tags

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSK15

Plasmid

#72855

Purpose

template for insertion of attP site (C-terminal tag plasmid design), Blasticidin-Resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

attP site

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pRB33

Plasmid

#72867

Purpose

template for N-terminal 3x FLAG tag, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

3xFlag tag

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSK25

Plasmid

#72853

Purpose

template for C-terminal double-FLAG tag, Puromycin-resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

double Flag

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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pAH233

Plasmid

#121144

Purpose

Expression of gRNA with BbsI placeholder for target cloning

Depositor

Katsunori Tanaka

Article

Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

Insert

gRNA cassette

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

S.pombe rrk1

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSK24

Plasmid

#72852

Purpose

template for C-terminal Twin-Strep tag, Puromycin-resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

twin Strep tag

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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pSK32

Plasmid

#72854

Purpose

template for C-terminal TEV-site + V5-tag, Puromycin-Resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

TEV-V5 tag

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pAH235

Plasmid

#121145

Purpose

Cas9 expression controlled by the nmt41 promoter

Depositor

Katsunori Tanaka

Article

Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

Insert

humanized Streptococcus pyogenes Cas9

Use

CRISPR

Tags

Flag

Expression

Yeast

Mutation

Promoter

nmt41

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRB31

Plasmid

#72868

Purpose

template for N-terminal 1x Strep tag, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

single Strep tag

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRB30

Plasmid

#72860

Purpose

template for N-terminal eGFP tag, inducible Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

eGFP

Use

Tags

Expression

Insect

Mutation

eGFP

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCU-LYS21

Plasmid

#45331

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Type

Empty backbone

Use

C. glabrata expression

Tags

Expression

Mutation

Promoter

LYS21

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pKF296

Plasmid

#74773

Purpose

template for C-terminal TEV-site + 2x V5-tag (short version), Blasticidin-Resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

TEV- 2xV5 tag (short version)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRB32

Plasmid

#72863

Purpose

template for N-terminal 1x Strep tag, constitutive Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

single Strep tag

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCU-ACO2

Plasmid

#45327

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Type

Empty backbone

Use

C. glabrata expression

Tags

Expression

Mutation

Promoter

ACO2

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA

Plasmid

#191528

Purpose

Vector for tandem expression of SLBP 3'UTR G1 sgRNA in combination with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate SLBP endogenous tagging by marker free coselection using ouabain

Depositor

Yannick Doyon

Insert

SLBP 3'UTR G1 sgRNA + ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR; Co-selection via hdr using ouabain

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR{KDRT-RC}

Plasmid

#182166

Purpose

pENTR plasmid carrying yeast recombination site for Gateway cloning into introns of genes encoding fluorescent proteins

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

KDRT

Use

Pentr plasmid

Tags

Expression

Mutation

Promoter

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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p{3P3-mTurquoise::FRT,attB}

Plasmid

#182180

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

mTurquoise

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{3P3-mTurquoise::GW,attB}

Plasmid

#182169

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

mTurquoise

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge