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Plasmid#171095Purposelentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2DepositorInsertOsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
UseLentiviral and Synthetic BiologyTagsEmerald, Geminin, miniAID, mCherry2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPtGE35
Plasmid#107999PurposeExpresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugationDepositorInsertsOriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3
I-TevI nuclease and partial linker domain
UseCRISPR and Synthetic Biology; Episomal vector for…TagsCas9ExpressionMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-NCOA7g2 (BB35)
Plasmid#139453PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUT1158-HQ858702
Plasmid#27748DepositorInsertG2-like transcription factor from EE295362
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceMarch 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pF1-Hs Cdk1/cyclin B1
Plasmid#177330PurposeCo-expression of the human active Cdk1/cyclin B1 kinase complexDepositorUseTags2 x Strep-tag and 8 x HisExpressionInsectMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGMC00035 (aka pMG0784)
Plasmid#210768PurposeLentiviral vector to express human ADRM1DepositorInsertADRM1 (ADRM1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_ORI_AGAP1
Plasmid#99300PurposeLuciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr2: 236735694-236737193 (AGAP1 Human)
UseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_mP_AGAP1
Plasmid#99301PurposeLuciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr2: 236735694-236737193 (AGAP1 Human)
UseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_SCP1_AGAP1
Plasmid#99302PurposeLuciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr2: 236735694-236737193 (AGAP1 Human)
UseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting
Plasmid#92139PurposeTargeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selectionDepositorUseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF2151
Plasmid#142920PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXG1 (FOXG1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pF1-Hs Cdk1/cyclin B1/Cks1
Plasmid#177329PurposeCo-expression of the human active Cdk1/cyclin B1/Cks1 kinase complexDepositorInsertsUseTags2 x Strep-tag and 8 x HisExpressionInsectMutationPromoterp10 and polhAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag CDC2
Plasmid#20446DepositorInsertCDC2 (CDK1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CDC2
Plasmid#23776DepositorInsertCDC2 (CDK1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only