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pEF1α-squirrel-monkey-retroCHMP3-T2A-H2B-BFP

Plasmid

#154185

Purpose

expresses squirrel monkey retroCHMP3 with self-cleaving tag in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Use

Tags

HA and T2A-H2B-BFP

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-MBD

Plasmid

#114371

Purpose

expresses Flpo recombinase-dependent GtACR2-MBD, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-MBD (NEWENTRY )

Use

AAV

Tags

EYFP and MBD

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-FLEX-rc [Archon1-KGC-GFP-ER2]

Plasmid

#115891

Purpose

AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

Promoter

EF1α promoter (1.1kb short version)

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJM582 EF1α 3xFLAG-NLS-VP64-ZF2 in TUPV2

Plasmid

#161543

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-ZF2 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-ZF2

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM465 EF1α 3xFLAG-NLS-VP64-ZF1 in TUPV2

Plasmid

#161533

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-ZF1 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-ZF1

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-TlcnC

Plasmid

#114374

Purpose

expresses Flpo recombinase-dependent GtACR2-TlcnC, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-TlcnC (NEWENTRY )

Use

AAV

Tags

EYFP and TlcnC

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pJM554 EF1α 3xFLAG-NLS-VP64-intN in TUPV2

Plasmid

#161556

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-intN under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-intN

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM555 EF1α 3xFLAG-NLS-DsDed-intN in TUPV2

Plasmid

#161557

Purpose

Constitutive expression of 3xFLAG-NLS-DsDed-intN under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-intN

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM583 EF1α 3xFLAG-NLS-VP64-ZF6 in TUPV2

Plasmid

#161544

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-ZF6 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-ZF6

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM506 EF1α 3xFLAG-NLS-DsDed-ZF1 in TUPV2

Plasmid

#161536

Purpose

Constitutive expression of 3xFLAG-NLS-DsDed-ZF1 gene under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-ZF1

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM507 EF1α 3xFLAG-NLS-DsDed-ZF10 in TUPV2

Plasmid

#161537

Purpose

Constitutive expression of 3xFLAG-NLS-DsDed-ZF10 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-ZF10

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM512 EF1α 3xFLAG-NLS-VP64-ZF1 in TUPV2

Plasmid

#161538

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-ZF1 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-ZF1

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM520 EF1α 3xFLAG-NLS-VP64-ZF10 in TUPV4

Plasmid

#161539

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-ZF10 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-ZF10

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv4.2LL

Plasmid

#114373

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv4.2LL, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv4.2LL (NEWENTRY )

Use

AAV

Tags

EYFP and Kv4.2LL

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C

Plasmid

#114375

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv2.1C, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv2.1C (NEWENTRY )

Use

AAV

Tags

EYFP and Kv2.1C

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Nlgn1C

Plasmid

#114372

Purpose

expresses Flpo recombinase-dependent GtACR2-Nlgn1C, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Nlgn1C (NEWENTRY )

Use

AAV

Tags

EYFP and Nlgn1C

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA

Plasmid

#107311

Purpose

Encoding scFv-p65-HSF1 driven by EF1α promoter

Depositor

Hui Yang

Article

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Insert

EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)

Plasmid

#197893

Purpose

Can be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

mCherry

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

EF1a

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)

Plasmid

#197892

Purpose

Can be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

hM4D/2A/GFP

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

EF1a

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC

Plasmid

#114376

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv2.1C-TlcnC (NEWENTRY )

Use

AAV

Tags

EYFP, Kv2.1C, and TlcnC

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge