Showing: 121 - 140 of 1875 results
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Plasmid#199253PurposeExpression construct encoding the high specificity SpCas9-KA-D10A nickaseDepositorInsertHigh specificity S. pyogenes Cas9-D10A-KA nickase
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10A K848APromoterCAG promoterAvailabilityAcademic Institutions and Nonprofits only -
mito-PDCD10-mScarlet-I
Plasmid#208887PurposeExpress mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.DepositorArticleInsertCCM3 (PDCD10 Human)
UseTagsmScarlet-IExpressionMammalianMutationPDCD10 targeted to the mitonchondriaPromoterAvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-eSpCas9-D10A
Plasmid#80449Purposeenhanced SpCas9 mammalian expression vectorDepositorInsertehSpCas9_D10A
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pHL-EF1a-SphcCas9(D10A)-iP-A
Plasmid#60600PurposeExpresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.DepositorInsertsCRISPR Cas9 D10A
puromycin resistance gene
UseCRISPRTagsExpressionMammalianMutationChanged Asp 10 to Ala, Codon usage optimized for …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCI-FLAG-mTUT4(D1028A)
Plasmid#60220PurposeMammalian expression of FLAG-tagged catalytic mutant of TUT4DepositorInsertTUT4 (Tut4 Mouse)
UseTagsFLAGExpressionMammalianMutationD1028APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 D102A, E108A
Plasmid#74618PurposeExpresses a double mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationD102A, E108APromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-nCas9(D10A)-PmCDA1
Plasmid#79617PurposeExpresses nCas9(D10A)-PmCDA1 in yeast cellsDepositorInsertSpCas9
UseTagsPmCDA1ExpressionYeastMutationD10A for nickase Cas9PromoterpGal1AvailabilityAcademic Institutions and Nonprofits only -
Top7 D9T D10V N14T
Plasmid#12473DepositorInsertTop7 D9T D10V N14T
UseTags6xHisExpressionBacterialMutationD9T D10V N14TPromoterAvailabilityAcademic Institutions and Nonprofits only -
GST-GW1D10(aa566-1343)
Plasmid#21516DepositorInsertTNRC6A (TNRC6A Human)
UseTagsGSTExpressionMammalianMutationcontain aa566-1343PromoterAvailabilityAcademic Institutions and Nonprofits only -
GFP-GW1D10(aa566-1343)
Plasmid#21517DepositorInsertTNRC6A (TNRC6A Human)
UseTagsGFPExpressionMammalianMutationcontain aa566-1343PromoterAvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐Afu‐rnhB D101N
Plasmid#108695PurposeFor expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutationDepositorInsertrnhB
UseTagsGSTExpressionBacterialMutationD101N catalytic site mutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_D1078A
Plasmid#91860PurposeBacterial expression for Cas13aDepositorInsertLbuCas13a (LbuC2c2) D1078A
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationD1078APromoterAvailabilityAcademic Institutions and Nonprofits only -
pPD1019 TRE3GV DsRedExpress2 in TU2
Plasmid#139254PurposeTRE3GV promoter and DsRedExpress2 in TUPV2DepositorInsertDsRedExpress2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GVAvailabilityAcademic Institutions and Nonprofits only -
pPD1023 TRE3GV DsRedExpress2 in TU5
Plasmid#139257PurposeTRE3GV promoter and DsRedExpress2 in TUPV5DepositorInsertDsRedExpress2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GVAvailabilityAcademic Institutions and Nonprofits only -
pPD1026 EF1alpha TetON3G in TU2
Plasmid#139258PurposeExpresses TetON3G under hEF1alpha in TUPV2DepositorInsertTetON3G
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
pPD1024 EF1alpha TetON3G in TU5
Plasmid#139261PurposeExpresses TetON3G under hEF1alpha in TUPV5DepositorInsertTetON3G
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
pPD1025 EF1alpha TetON3G in TU6
Plasmid#139262PurposeExpresses TetON3G under hEF1alpha in TUPV6DepositorInsertTetON3G
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
pPD107 VP16-ZF43(RARR)
Plasmid#138812PurposeConstitutive expression of VP16-ZF43(RARR) under the CMV promoter.DepositorInsertVP16-ZF43(RARR)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR15APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pPD108 VP16-ZF43(RAAR)
Plasmid#138813PurposeConstitutive expression of VP16-ZF43(RAAR) under the CMV promoter.DepositorInsertVP16-ZF43(RAAR)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR15A, R43APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pPD109 VP16-ZF43(RAAA)
Plasmid#138814PurposeConstitutive expression of VP16-ZF43(RAAA) under the CMV promoter.DepositorInsertVP16-ZF43(RAAA)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR15A, R43A, R71APromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 121 - 140 of 1875 results