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Plasmid#210652PurposeIn vitro expression of polyProline FETCH reporter from the T7 promoterDepositorInsertsFLAG-p1-PRB4_R7-9-FETCH1
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sFLAG-a5-PRB4_R7-9A-FETCH1
Plasmid#210653PurposeIn vitro expression of polyProline FETCH control from the T7 promoterDepositorInsertsFLAG-a5-PRB4_R7-9A-FETCH1
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Notch1 [N253/32R]
Plasmid#206549PurposeMammalian Expression Plasmid of anti-Notch1 (Mouse). Derived from hybridoma N253/32.DepositorInsertanti-Notch1 (Mus musculus) recombinant Mouse monoclonal antibody (Notch1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACH1-2EGFP-ter-URA3
Plasmid#184769PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertACH1 (ACH1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV sgRNA-Notch1 #2 GFP
Plasmid#106951PurposeLentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.DepositorInsertgRNA targeting Notch1 (Notch1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV sgRNA-Notch1 #3 GFP
Plasmid#106952PurposeLentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.DepositorInsertgRNA targeting Notch1 (Notch1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-CH111-QES.c13.L544Y
Plasmid#123267PurposeMammalian expression plasmid for Env from the CH111 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopesDepositorInsertHIV-1 (AD8) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Mutations V208M, …PromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCh1750_S2F-IMCg_doxy-CMV_mChe-Cdc42E7
Plasmid#118614Purposeexpression of mChe-tagged cdc42E7 under doxy-inducible CMV promoterDepositorInsertcdc42E7 (Cdc42 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationPromotertetON CMVAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-V111FL114FI766F-HA-TagBFP
Plasmid#120911PurposeExpresses BFP tagged Ptch1-B (V111FL114FI766F)DepositorInsertPtch1 (Ptch1 Mouse)
UseTagsHA and TagBFPExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291, Y…PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-V111FL114FW115A-HA-GFP
Plasmid#120908PurposeExpresses Ptch1-B (V111FL114FW115A)DepositorInsertPtch1 (Ptch1 Mouse)
UseTagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-I766FV111F-HA-GFP
Plasmid#120907PurposeExpresses Ptch1-B (I766FV111F)DepositorInsertPtch1 (Ptch1 Mouse)
UseTagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOZ-FH-C Bach1 K141A/K142A
Plasmid#73646PurposeRetroviral expression of Bach1 K141A/K142A mutant (mlh1 binding mutant)DepositorInsertBach1 (BACH1 Human)
UseRetroviralTagsFLAG and HisExpressionMammalianMutationK141A/K142A ( mlh1 binding mutant)PromoterunknownAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 Bach1 K141A/K142A
Plasmid#73642PurposeMammalian expression of Bach1 K141A/K142A mutant (mlh1 binding mutant)DepositorInsertBach1 (BACH1 Human)
UseTagsHis and MycExpressionMammalianMutationK141A/K142A ( mlh1 binding mutant)PromoterCMVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
RCH1:HDT1,HDT2-RNAi
Plasmid#108446PurposeArabidopsis transformation, RNAi vector targeting HDT1/HDT2 with RCH1 promoterDepositorUseRNAiTagsExpressionPlantMutationPromoterRCH1Available sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Notch1 Full Length-6MT
Plasmid#41728DepositorInsertNotch-1 Full Length (Notch1 Mouse)
UseTags6xMycExpressionMammalianMutationL1997V; Contains murine Notch-1 protein up to ami…PromoterAvailable sinceDec. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato
Plasmid#113865PurposeTriple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.DepositorInsertsCMV-eGFP
TOP-iRFP
PTCH1-mTurquoise2
CBF-tdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1)
Plasmid#217340PurposecrRNA array targeting CD81, B2M, KIT, CD55DepositorInsertsUseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCD5-H3CH13-CO-GCN4-TEV-sfGFP-TS
Plasmid#158740PurposeExpresses influenza A virus trimeric hemagglutinin A/H3/CH/13 fused to a sfGFPDepositorInsertHemagglutinin A/MI/45/15 H1N1
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutationAA61-469PromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-his-BACH1 K52R
Plasmid#17643DepositorInsertBACH1 (BRIP1 Human)
UseTagsHis and MycExpressionMammalianMutationThe lysine residue at position 52 was changed to …PromoterAvailable sinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
gWiz_BM40-vGAT-VH-hIgG1-CH1-TS-HIS
Plasmid#218436Purposemammalian expression plasmid for the heavy chain fragment of an anti-vGAT Fab domainDepositorInsertvGAT-Vh-hIgG1-CH1
UseAffinity Reagent/ AntibodyTagsBM40 and TwinStrep-6xHisExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only