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Djun - antisense

Plasmid

#38338

Depositor

Norbert Perrimon

Article

Zhang et al Proc Natl Acad Sci U S A. 1990 Aug;87(16

Insert

djun (Jra Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Hsp70

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
cmv 16 E7 Q mutant

Plasmid

#13696

Depositor

Karl Munger

Insert

HPV 16 E7 Q mutant (E7 Human)

Use

Tags

Expression

Mammalian

Mutation

aa 30-37 changed from DSSEEEDE to QSSQQQQQ.

Promoter

Available since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
Venus-giantin

Plasmid

#234749

Purpose

Mammalian expression of Golgi marker Venus-giantin

Depositor

Nevin Lambert

Article

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Insert

Venus-giantin

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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env8-AD-5'antiPNA-U1

Plasmid

#233011

Purpose

U1 snRNA tagged with 1 copy of Riboglow (truncated variant A with 5' antiPNA motif)

Depositor

Amy Palmer

Article

Wierzba et al RSC Chem Biol. 2024 Dec 19. doi: 10.1039

Insert

U1 (RNU1-1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 copy of Riboglow tag (truncated variant A with …

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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fabp10a:H2B-mGreenLantern

Plasmid

#230045

Purpose

Expression of nuclear green fluorescent protein in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Insert

mGreenLantern

Use

Zebrafish expression

Tags

H2B

Expression

Mutation

Promoter

fabp10a

Available since

Feb. 5, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Anti-Erbin [L4/6R]

Plasmid

#227019

Purpose

Mammalian expression plasmid of anti-Erbin (Human) IgG2a R-mAb. Derived from hybridoma L4/6

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Erbin (Homo sapiens) recombinant mouse monoclonal antibody. (ERBIN Mouse)

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

Plasmid

#191572

Purpose

Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

SARS-CoV-2 Spike Protein (S Synthetic)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

D614G and F486S (confers reduced binding affinity…

Promoter

CMV

Available since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pL0_CD-greenlantern

Plasmid

#202141

Purpose

Level 0 plasmid containing a green lantern fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Green lantern gene

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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GPI-mGreenLantern(2x)

Plasmid

#197917

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mGreenLantern-mGreenLantern

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_105 [tAnTrpC]

Plasmid

#199006

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

tAnTrpC

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_104 [pAnTrpC]

Plasmid

#199005

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAnTrpC

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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H5_fold-0 _Chantal

Plasmid

#201826

Purpose

Expresses H5_fold-0_Chantal in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H5_fold-0 _Chantal

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD8alpha variant 3

Plasmid

#188363

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant3-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant3-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_WT_1kb

Plasmid

#194187

Purpose

Includes the promoter (1kb) of SMTS (SMANTIS, MANTIS, AK125871)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

SMANTIS promoter (1kb)

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-mGreenLantern332

Plasmid

#188483

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.

Depositor

Johan Sosa, Indeever Madireddy

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern sgRNA

Use

CRISPR

Tags

PB_rtTA_BsmBI

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_5 (pBS0722)

Plasmid

#185225

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_5. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_5

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_3 (pBS0720)

Plasmid

#185224

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_23 (pBS0719)

Plasmid

#185223

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_23. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_23

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_pvLEA22mer_mutant_22 (pBS0718)

Plasmid

#185222

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_22. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_22

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_21 (pBS0717)

Plasmid

#185221

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_21. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_21

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge