1. pSpCas9(BB)-2A-GFP (PX458)

   Plasmid

   #48138

   Purpose

   Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

   Plasmid

   #129030

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA2 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-3

   Plasmid

   #129031

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA3 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA3 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

   Plasmid

   #129032

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA4 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CCND2 targeting gRNA

   Plasmid

   #215318

   Purpose

   Expresses gRNA targeting CCND2 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CCND1 targeting gRNA

   Plasmid

   #215153

   Purpose

   Expresses gRNA targeting CCND1 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75236

   Purpose

   CRISPR/Cas9 plasmid against human NFATc1

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc1 (NFATC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75237

   Purpose

   CRISPR/Cas9 plasmid against human NFATc1

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc1 (NFATC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75244

   Purpose

   CRISPR/Cas9 plasmid against human Helios

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Helios (IKZF2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)

   Plasmid

   #171099

   Purpose

   CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9 (Ascl1 S. pyogenes, Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKG210

    Plasmid

    #69761

    Purpose

    Retroviral expression of CENP-T aa 1-375

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 1-375 (CENPT Human)
    

    Use

    Retroviral

    Tags

    LacI-NLS and mCherry

    Expression

    Mammalian

    Mutation

    e.coli optimized sequence

    Promoter

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pKG212

    Plasmid

    #69758

    Purpose

    Retroviral expression of CENP-T aa 1-242

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 1-242 (CENPT Human)
    

    Use

    Retroviral

    Tags

    LacI-NLS and mCherry

    Expression

    Mammalian

    Mutation

    e.coli optimized sequence

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pKG402

    Plasmid

    #69760

    Purpose

    Retroviral expression of CENP-T aa 256-561

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 256-561 (CENPT Human)
    

    Use

    Retroviral

    Tags

    mCherry-TEV-S

    Expression

    Mammalian

    Mutation

    e.coli optimized sequence

    Promoter

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pKG194

    Plasmid

    #69757

    Purpose

    Mammalian Expression of CENP-T aa 1-375

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 1-375 (CENPT Human)
    

    Use

    Retroviral

    Tags

    LacI-NLS and eGFP

    Expression

    Mammalian

    Mutation

    e.coli optimized sequence

    Promoter

    Available since

    Nov. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pKG326

    Plasmid

    #69753

    Purpose

    Retroviral expression of CENP-T aa 1-531

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 1-531 (CENPT Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Mutation

    E.coli optimized sequence; A216T and G459R mutati…

    Promoter

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pGS275_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)]

    Plasmid

    #211891

    Purpose

    Bacterial expression of CENP-T[1-242/3D]

    Depositor

    Iain Cheeseman

    Insert

    CENP-T[1-242/3D] (CENPT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T11D, T27D, T85D

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGS58_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)] - I3-01

    Plasmid

    #211890

    Purpose

    Bacterial expression of CENP-T[1-242/3D]-I3-01

    Depositor

    Iain Cheeseman

    Insert

    CENP-T[1-242/3D]-I3-01 (CENPT Synthetic, Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T11D, T27D, T85D

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKG113

    Plasmid

    #63073

    Purpose

    Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    CENP-T (CENPT Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Mutation

    E. coli codon optimized. G217R mutation compared …

    Promoter

    Available since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGS97_EGFP(A206K)-CENP-T(1-242)-I3-01 AAVS donor

    Plasmid

    #211866

    Purpose

    CENP-T(1-242)-I3-01 transgene integration at AAVS1 safe harbor locus

    Depositor

    Iain Cheeseman

    Insert

    CENP-T(1-242)-I3-01 (CENPT Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGS101_EGFP(A206K)-CENP-T(1-242) AAVS donor

    Plasmid

    #211867

    Purpose

    CENP-T(1-242) transgene integration at AAVS1 safe harbor locus

    Depositor

    Iain Cheeseman

    Insert

    CENP-T(1-242) (CENPT Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ZJOM160

    Plasmid

    #133670

    Purpose

    Integration of mTagBFP2-SKL as an additional copy in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast peroxisome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-mTagBFP2-SKL
    

    Use

    Tags

    mTagBFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge