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Plasmid#201872PurposeUsed for bacterial expression of human protein ARL8ADepositorInsertARL8A (ARL8A Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA_mouse_Adar2_Tv1
Plasmid#158110Purposeexpresses mADAR2 shortest transcriptDepositorInsertmAdar2 (Adarb1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Scarlet-Kif5a
Plasmid#118745PurposeExpresses scarlet tagged Kif5aDepositorInsertKif5a cDNA (Kif5a Mouse)
UseTagsmScarletExpressionMammalianMutationPromoterCAGAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pScarlet-Kif5a
Plasmid#118744PurposeExpresses scarlet tagged Kif5aDepositorInsertKif5a cDNA (Kif5a Mouse)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS_MmaPylT_EF1_NES-MmaPylRS WT
Plasmid#174901Purposeexpression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAG and NESExpressionMammalianMutationPromoterEF1Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Tet1
Plasmid#60938PurposeExpresses Tet1 in mammalian cellsDepositorInsertTen-Eleven Translocation 1 (Tet1 Mouse)
UseTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS_MmaPylT_EF1_NES-MmaPylRS AF
Plasmid#174902Purposeexpression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAG and NESExpressionMammalianMutationY306A, Y384FPromoterEF1Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG172-STII PLU-HIS
Plasmid#112992PurposeBaculovirus expression of Drosophila png172-Flag and plu-HisDepositorUseTagsFlag and HisExpressionInsectMutationpng172 mutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG-STII PLU-HIS
Plasmid#112993PurposeBaculovirus expression of Drosophila png-Flag and plu-HisDepositorUseTagsFlag and HisExpressionInsectMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS_sfGFP150K
Plasmid#193313PurposepAS control plasmid for sfGFP expressionDepositorInsertsuper folder GFP
UseTagsExpressionMammalianMutation150K in sfGFPPromoterEF1Available sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
dishevelled
Plasmid#38342DepositorInsertdishevelled (dsh Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTCW002
Plasmid#83522Purposepheromone mediated destablised-GFP expression in yeastDepositorInsertpFUS1-yEGFPCLN2PEST
UseTagsExpressionYeastMutationPromoterpFUS1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB_SBE4:mScarlet-I-NLS
Plasmid#154961Purposeencodes transcriptional reporter of SMAD2/3DepositorInsertmScarlet
UsePiggybacTagsNLS-PESTExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
MK1283
Plasmid#71428PurposeThis E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709DepositorInsertMixed Feedback Loop version of the UBER system
UseTagsExpressionMutationT7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …PromoterAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
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MK1274
Plasmid#71431PurposeThis E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.DepositorInsertMixed Feedback Loop version of the UBER system
UseTagsExpressionMutationT7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…PromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pH3U3-zif268 (omega)
Plasmid#18046DepositorInsertzif268 binding site
UseTagsExpressionBacterialMutationThe preferred binding site for zif268 is inserted…PromoterAvailable sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pTCW007
Plasmid#83525Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoterDepositorInsertpFUS1J2-mfalpha1
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW008
Plasmid#83526Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoterDepositorInsertpFUS1J2-mfalpha2
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only