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Showing: 301 - 350 of 2164 results

  22. pSBtet-2xMARS-nGFP-puro

    Plasmid
    #205239
    Purpose
    DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system
    Depositor
    Jeremy Baskin
    Insert
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    Use
    Transposon
    Tags
    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    tight TRE promoter
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205239-plasmid-map-sequence-id-404740

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  32. GFP-ATG8(416)/GFP-AUT7(416)

    Plasmid
    #160389
    Purpose
    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selection
    Depositor
    John McCusker
    Insert
    autophagy-related 8 (ATG8 Budding Yeast)
    Use
    Cen/ars
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Endogenous ATG8 promoter
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160389-plasmid-map-sequence-id-314524

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  37. GFP-LiMETER-v2

    Plasmid
    #113934
    Purpose
    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP
    Depositor
    Yubin Zhou
    Article
    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi
    Insert
    LOV2 and Stim1 polybasic domain (PB)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    113934-plasmid-map-sequence-id-225307

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  38. EGFP–COSMO–PB

    Plasmid
    #169684
    Purpose
    COSMO responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Use
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y104W
    Promoter
    CMV
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    169684-plasmid-map-sequence-id-338138

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  39. EGFP–acVHH–PB

    Plasmid
    #169685
    Purpose
    An anti-caffeine nanobody responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody
    Use
    Tags
    modified STIM1-PB tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    169685-plasmid-map-sequence-id-338140

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  49. LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid
    #48253
    Purpose
    LITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    CRY2PHR-NLS-VP64
    Use
    AAV; Tale
    Tags
    2A_GFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-alpha
    Available since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    48253-plasmid-map-sequence-id-70481

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Showing: 301 - 350 of 2164 results