Addgene

We narrowed to 2,025 results for: CO

Showing: 301 - 350 of 2025 results

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Flag-hPIASx alpha

Plasmid

#15209

Depositor

Ke Shuai

Article

Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

Insert

PIASx alpha (PIAS2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
PB-CAG-GFPd2

Plasmid

#115665

Purpose

Piggybac transposon plasmid for destabilized eGFP (GFPd2)

Depositor

Jordan Green

Article

Rui et al ACS Appl Mater Interfaces. 2019 Feb 22.

Insert

GFPd2

Use

Synthetic Biology

Tags

Destabilization

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 EGFP

Plasmid

#115786

Purpose

Expresses EGFP in mammalian cells

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKD227

Plasmid

#90954

Purpose

Expresses S. baltica TtrS under the Ptac promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

Tetrathionate sensor kinase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCFD3-ebony

Plasmid

#83380

Purpose

Drosophila gRNA expression plasmid targets ebony

Depositor

Richard Padgett

Article

Kane et al G3 (Bethesda). 2017 Jan 5;7(1):87-93. do

Insert

ebony (e Fly)

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST47-SAR1

Plasmid

#67472

Purpose

Mammalian expression of hSar1 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pMLM802

Plasmid

#27203

Depositor

Keith Joung

Article

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pMLM800

Plasmid

#27202

Depositor

Keith Joung

Article

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST47-SARA

Plasmid

#67473

Purpose

Mammalian expression of hSara with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
piggybac-EF1-halo-mTBP

Plasmid

#197908

Purpose

single-particle tracking for mTBP

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

TBP (Tbp Mouse)

Use

Tags

Halotag

Expression

Mammalian

Mutation

Promoter

EF1

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pTAG1

Plasmid

#176863

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_1

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSUP202pol4

Plasmid

#67184

Purpose

Conjugative cloning vector

Depositor

Julia Vorholt, Hauke Hennecke

Article

Fischer et al EMBO J. 1993 Jul;12(7):2901-12.

Type

Empty backbone

Use

Conjugative bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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pJC225

Plasmid

#176883

Purpose

MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_A5803

Plasmid

#176878

Purpose

High constitutive expression of Firefly Luciferase in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

CmYLCV:Luc+:HSP

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAG2E

Plasmid

#176865

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2E

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB1751

Plasmid

#174013

Purpose

pDUAL plasmid expressing GFP from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

GFP

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

41nmt1 and 41nmt1 promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntr-SRFflbio

Plasmid

#32971

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

SRF (SRF Human)

Use

Gateway

Tags

flag-bio

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-μ2-(HA)3

Plasmid

#198178

Purpose

Expression of HA-tagged AP-2 μ2 in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-2 μ2

Use

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitution in codon 80

Promoter

CMV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_D5802

Plasmid

#176880

Purpose

High constitutive expression of Firefly Luciferase in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

AtUbi10:Luc+:RbcsE9

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAJ08

Plasmid

#14912

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis actin promoter

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV0-OCT-HA-eGFP

Plasmid

#67479

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-SAR1

Plasmid

#67344

Purpose

hSar1 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pAJ50

Plasmid

#14918

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

enhanced monomeric red fluorescent protein

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST14-SAR1

Plasmid

#67388

Purpose

Expression of native hSar1 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pAJ20

Plasmid

#14919

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

Strongyloides stercoralis Ribosomal protein small subunit

Use

Tags

GFP(S65C)

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV1-OCT-HA-eGFP

Plasmid

#67480

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

attenuated CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pAJ09

Plasmid

#14913

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis G protein alpha subunit promoter

Use

Tags

GFP(S65C)

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-OCT-HA-eGFP

Plasmid

#67485

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

attenuated CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCMV4-OCT-HA-eGFP

Plasmid

#67483

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

attenuated CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCMV2-OCT-HA-eGFP

Plasmid

#67481

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

attenuated CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-SARA

Plasmid

#67346

Purpose

hSara with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCCM937

Plasmid

#58982

Purpose

Expresses avr-15 sgRNA in C.elegans.

Depositor

Craig Mello

Article

Kim et al Genetics. 2014 May 30. pii: genetics.114

Insert

avr-15 sgRNA

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pCCM936

Plasmid

#58981

Purpose

Expresses avr-14 sgRNA in C.elegans.

Depositor

Craig Mello

Article

Kim et al Genetics. 2014 May 30. pii: genetics.114

Insert

avr-14 sgRNA

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pAJ13

Plasmid

#14915

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

multiple cloning site 3

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS458-U6_CBA-mCherry

Plasmid

#195566

Purpose

Transient expression of mCherry; U6 with scaffold for sgRNA cloning in mammalian cells.

Depositor

Joanna Wysocka

Article

Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-μ3A-(HA)3

Plasmid

#198179

Purpose

Expression of HA-tagged AP-3 μ3A in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-3 μ3A

Use

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitution in codon 225

Promoter

CMV

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_C'4800EC

Plasmid

#176875

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_A5801

Plasmid

#176877

Purpose

High constitutive expression of Firefly Luciferase in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

35S:Luc+:HSP

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAG4E

Plasmid

#176868

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4E

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAG5

Plasmid

#176869

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_5

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAG6E

Plasmid

#176870

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_6E

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-1-CmYLCV-6

Plasmid

#176872

Purpose

MoClo Level 0 plasmid, contains CmYLCV-6 promoter

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

CmYLCV

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAG2

Plasmid

#176864

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAG3

Plasmid

#176866

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_3

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAG4

Plasmid

#176867

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
kank1b_pJet1.2

Plasmid

#121979

Purpose

for generating kank1b riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank1b (si:dkey-226m8.9 Zebrafish)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-OCT-HA-eGFP

Plasmid

#67484

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

attenuated CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCMV3-OCT-HA-eGFP

Plasmid

#67482

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

attenuated CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-SARA

Plasmid

#67389

Purpose

Expression of native hSara in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pAJ12

Plasmid

#14914

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

multiple cloning site 3

Use

Tags

GFP(S65C)

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 301 - 350 of 2025 results

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