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Showing: 301 - 350 of 1133 results

  1. U6m

    Plasmid
    #121019
    Purpose
    MoClo golden gate assembly BC part for BCD20 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD20
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121019-plasmid-map-sequence-id-238082

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  2. U8m

    Plasmid
    #121021
    Purpose
    MoClo golden gate assembly BC part for BCD24 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD24
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121021-plasmid-map-sequence-id-236184

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  3. pcDNA3-TLR2-YFP

    Plasmid
    #13016
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 2 (TLR2 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13016-plasmid-map-sequence-id-7416

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  4. pcDNA3-TLR2-CFP

    Plasmid
    #13015
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 2 (TLR2 Human)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13015-plasmid-map-sequence-id-7415

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  5. pcDNA3-TLR4-YFP

    Plasmid
    #13018
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 4 (TLR4 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13018-plasmid-map-sequence-id-339915

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  6. pcDNA3-TLR1-YFP

    Plasmid
    #13014
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 1 (TLR1 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13014-plasmid-map-sequence-id-237573

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  7. U24m

    Plasmid
    #121026
    Purpose
    MoClo golden gate assembly BC part for RiboJ-BCD2 (low expression bi-cistronic RBS, engineered for context-independence, prefixed by self-cleaving RiboJ; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    RiboJ + BCD2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121026-plasmid-map-sequence-id-238071

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  8. pCDNA3-hTLR4-VenusFlag

    Plasmid
    #197640
    Purpose
    Human TOLL protein expression in mammalian cells
    Depositor
    Haian Fu
    Insert
    TLR4 (TLR4 Human)
    Use
    Tags
    Venus-Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197640-plasmid-map-sequence-id-391702

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  9. pDEST26-hTLR4-Nluc

    Plasmid
    #197642
    Purpose
    Human TOLL protein expression in mammalian cells
    Depositor
    Haian Fu
    Insert
    TLR4 (TLR4 Human)
    Use
    Tags
    NanoLuciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197642-plasmid-map-sequence-id-392621

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  10. pDEST26-Nluc-hTLR4

    Plasmid
    #197641
    Purpose
    Human TOLL protein expression in mammalian cells
    Depositor
    Haian Fu
    Insert
    TLR4 (TLR4 Human)
    Use
    Tags
    NanoLuciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197641-plasmid-map-sequence-id-392580

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  11. g5s pD47

    Plasmid
    #117451
    Purpose
    Expression of Cyth2
    Depositor
    Richard Kahn
    Insert
    Cyth2 (CYTH2 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    117451-plasmid-map-sequence-id-226803

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  12. g5s pD42

    Plasmid
    #117452
    Purpose
    Expression of Cyth2
    Depositor
    Richard Kahn
    Insert
    Cyth2 (CYTH2 Human)
    Use
    Tags
    V5-His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    117452-plasmid-map-sequence-id-226811

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  13. g5s pDONR

    Plasmid
    #117450
    Purpose
    Gateway Vector for Cyth2
    Depositor
    Richard Kahn
    Insert
    Cyth2 (CYTH2 Human)
    Use
    Gateway shuttling vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    117450-plasmid-map-sequence-id-226801

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  14. R777-E031 Hs.CYTH2

    Plasmid
    #70315
    Purpose
    Gateway ORF clone of human CYTH2 [NM_017457.5] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    CYTH2 (CYTH2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70315-plasmid-map-sequence-id-125764

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  15. R777-E032 Hs.CYTH2-nostop

    Plasmid
    #70316
    Purpose
    Gateway ORF clone of human CYTH2 [NM_017457.5] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    CYTH2 (CYTH2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70316-plasmid-map-sequence-id-125765

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  18. AA498

    Plasmid
    #231119
    Purpose
    Fragmid fragment: (guide cassette) ABE activity-based selection CD274 positive control
    Depositor
    David Root, John Doench
    Insert
    sgCD274 + ABE splice-targeting sgRNA
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231119-plasmid-map-sequence-id-458365

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  19. AA505

    Plasmid
    #231120
    Purpose
    Fragmid fragment: (guide cassette) CBE activity-based selection CD274 positive control
    Depositor
    David Root, John Doench
    Insert
    sgCD274 + CBE splice-targeting sgRNA
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231120-plasmid-map-sequence-id-458364

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  25. pCD86-mEGFP

    Plasmid
    #190745
    Purpose
    Expression of mEGFP-tagged CD86 in mammalian cells.
    Depositor
    Dave Piston
    Article
    Foust et al bioRxiv 2021.11.03.467169
    Insert
    CD86 B7-2, B7.2, B70, CD28LG2, LAB (CD86 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    190745-plasmid-map-sequence-id-373333

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  28. pcDNA5/FRT/TO_eGFP-pScaC-MTS

    Plasmid
    #228827
    Purpose
    For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC C-terminally fused to a mitochondrial targeting sequence in HeLa Flp-In cells
    Depositor
    Andrew Carter
    Article
    Manigrasso et al bioRxiv 2024.04.11.589026
    Insert
    ScaC
    Use
    Tags
    MTS and eGFP
    Expression
    Mammalian
    Mutation
    aa 33-223
    Promoter
    Available since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    228827-plasmid-map-sequence-id-456404

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  37. HSD17B10

    Plasmid
    #39153
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD17B10 (HSD17B10 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  38. pS44i8GM

    Plasmid
    #207527
    Purpose
    Conditionally-replicating in Pseudomonas vector, medium-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207527-plasmid-map-sequence-id-409603

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  39. MSCV-myrAkt

    Plasmid
    #49267
    Purpose
    retroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequence
    Depositor
    Arthur Weiss, Lawrence Kane
    Article
    Kane et al Nat Immunol. 2001 Jan;2(1):37-44.
    Insert
    Akt (AKT1 Bovine)
    Use
    Retroviral
    Tags
    IRES-EGFP and myr
    Expression
    Mammalian
    Mutation
    N2D, D149E and A478G compared to AAW71957.1
    Promoter
    Available since
    Jan. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  46. pJ02B2Gm:Rm_AF

    Plasmid
    #66061
    Purpose
    MoClo Device: FACS Standard Color Controls - High GFP (first unit) & RFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E:J23102:B:BCD2:C:E1010m:D:B0015:F]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    FACS Controls
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66061-plasmid-map-sequence-id-257697

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  47. pJ02B2Rm:Gm_AF

    Plasmid
    #66062
    Purpose
    MoClo Device: FACS Standard Color Controls - High RFP (first unit) & GFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E:J23102:B:BCD2:C:E0040m:D:B0015:F]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    FACS Controls
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66062-plasmid-map-sequence-id-257698

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Showing: 301 - 350 of 1133 results