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pLV-MPAct-mRuby3-IRES-Puro

Plasmid

#155231

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mRuby sensor

Use

Lentiviral

Tags

mRuby3

Expression

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pML104-HygMx4

Plasmid

#83475

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.

Depositor

Patricia Wittkopp

Insert

HphMX4

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pDDGFP_LEU2D

Plasmid

#58352

Purpose

Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

Depositor

Simon Newstead

Article

Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

Type

Empty backbone

Use

Tags

GFP-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE17

Plasmid

#90359

Purpose

NFAT - PKC/Ca++ gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

NFAT

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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TOPO mKate2

Plasmid

#68441

Purpose

TOPO construct expressing mKate2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mKate2

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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CMV10-3xFlag Skp2 WT

Plasmid

#81115

Purpose

mammalian expression of Skp2 WT

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

SKP2 (SKP2 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pGL-FLKif3A

Plasmid

#13742

Depositor

Linda Wordeman

Article

Ginkel et al Mol Biol Cell. 2000 Dec . 11(12):4143-58

Insert

mouse Kif3A (Kif3a Mouse)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2007

Availability

Academic Institutions and Nonprofits only

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pKM496 (deletions) Zeo

Plasmid

#109301

Purpose

ORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

Bxb1 attB site

Use

Orbit integrating plasmid

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-U6-dgRNA-CAG-MPH

Plasmid

#106259

Purpose

Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

MS2-P65-HSF1 (HSF1 Synthetic, Human)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-CECR2

Plasmid

#65385

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

CECR2 (CECR2 Human)

Use

Tags

EmGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only