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Plasmid#153482PurposeMammalian expression of FLAG-tagged Tapasin G15LDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationG15LPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin L18E
Plasmid#153483PurposeMammalian expression of FLAG-tagged Tapasin L18EDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationL18EPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin L18G
Plasmid#153484PurposeMammalian expression of FLAG-tagged Tapasin L18GDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationL18GPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin L18K
Plasmid#153485PurposeMammalian expression of FLAG-tagged Tapasin L18KDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationL18KPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT
Plasmid#141309PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CTDepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasinPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT E102A
Plasmid#153473PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT E102ADepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, E102APromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT M122A
Plasmid#153474PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT M122ADepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, M122APromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT G212A
Plasmid#153475PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT G212ADepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, G212APromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1371-sgGAL4
Plasmid#121514PurposesgGAL4 control. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.DepositorInsertsgGAL4
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromotermouse U6Available sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-Craf SSAA
Plasmid#118336PurposeExpression of human CrafDepositorInsertCraf SSAA (RAF1 Human)
UseTagsFlagExpressionMammalianMutationSSAAPromoterCMVAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
NET31 mRFP-N2 (490)
Plasmid#61984Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET31 (TMEM209 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EngHD
Plasmid#64033PurposeMammalian expression of TTF-1 homeodomain fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)DepositorInsertTTF-1 (TTF1 dog)
UseTagsEng (N-terminal repressor domain of the drosophi…ExpressionMammalianMutationhomeodomain, aa 154-226PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-TET1-Hygro
Plasmid#104404Purposetransient expression of dCas9-TET1 fusion proteinDepositorInsertTET1 (TET1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-dTET1-H1672Y-D1674A-Hygro
Plasmid#104413Purposetransient expression of dCas9-dTET1 fusion proteinDepositorInsertdTET1
UseCRISPRTagsExpressionMammalianMutationH1672Y-D1674APromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-JMJD2A-S288G-T289G-Hygro
Plasmid#104418Purposetransient expression of dCas9-dJMJD2A fusion proteinDepositorInsertdJMJD2A
UseCRISPRTagsExpressionMammalianMutationS288G-T289GPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA Display Part 1-4 DO
Plasmid#206514PurposeCcdB Dropout vector for parts 1-4 of Hemagglutinin surface expression in mammalian cellsDepositorTypeEmpty backboneUseTags3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…ExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas12Max-ABE
Plasmid#195335Purposevector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized dCas12Max-ABE
UseTagsExpressionMammalianMutationN243R, E336R, D1049APromoterCBh, CMV, hU6Available sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas12Max-CBE
Plasmid#195337Purposevector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized dCas12Max-CBE
UseTagsExpressionMammalianMutationN243R, E336R, D1049APromoterCBh, CMV, hU6Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
hfCas12Max
Plasmid#195336Purposevector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized hfCas12Max
UseTags3xFlagExpressionMammalianMutationN243R, E336R, D892RPromoterCBh, CMV, hU6Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Lifeact-EGFP-2XNLS
Plasmid#58467PurposeExpresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoterDepositorInsertsLifeact
2X NLS with 2X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only