-
Plasmid#98277Purposemammalian expression of DpsTE-YpetDepositorInsertDps
UseTagsYpetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
ITPKA_3xmTurquoise
Plasmid#112961PurposeIn vivo visualization of F-actin (can be used for colocalization studies)DepositorArticleInsertITPKA
UseTags3x mTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cip-pAct1-Mnase
Plasmid#162556PurposeNheI-MluI-digested 3xFLAG-MNase-SV40 fragment was cloned into the CIp-pACT1-CYC vector to ensure constitutive expression of MNase in C. albicans.DepositorInsert3FLAG-Mnase-SV40
UseTagsExpressionYeastMutationPromoterActineAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha1-iLifeact-NeonGreen
Plasmid#175258PurposeF-actin structures labellingDepositorInsertiLifeact
UseTagsmNeonGreenExpressionMammalianMutationNone (wt)PromoterpTub-alpha1Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEos2-VASP-5
Plasmid#57433PurposeLocalization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertVASP
UseTagsmEos2ExpressionMammalianMutationA209T in VASPPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
C453-E02: attB4-CAGp>-attB5
Plasmid#162972PurposeGateway attB4/attB5 entry clone for mammalian expression using the CAG promoterDepositorInsertchicken beta-actin/CMV enhancer promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pGBT-PX
Plasmid#31824DepositorTypeEmpty backboneUseCre/Lox; Gene trapTagsGFP GM2 variantExpressionMutationPromoterbeta ActinAvailable sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
ReaLiSM A1-Hinge-GPIba+2cys
Plasmid#59581Purposeexpress VWF A1-GPIba ReaLiSM construct in mammalian cellsDepositorInsertVWF A1-linker-GPIba (VWF Human)
UseTags6HisExpressionMammalianMutationPromoterchick beta-actinAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-rat PKA-RIIbeta
Plasmid#45529DepositorInsertPKA regulatory subunit RII beta
UseWith chicken beta actin promoter and wpreTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
-
pCAGGS-PHOSPHO2-TEV-Twin-Strep
Plasmid#222016PurposeExpress human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cellDepositorInsertPHOSPHO2 (PHOSPHO2 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TAA)Promoterchicken β-actin promoterAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2
Plasmid#227775PurposeExpression of beta actin driven BFP with multiple cloning site at 3' end. Also contains heat-shock activated mCherry reporter and CreERT2.DepositorInsertsBFP-MCS
mCherry-t2A-CreERT2
UseZebrafish expressionTagsExpressionMutationPromoterOlactb and hsp70lAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
p140Tol2-Olactb:SABER
Plasmid#226829PurposeExpression of beta actin driven BFP and molecular barcode array for SABER-seq in zebrafish. Also contains heat-shock activated mCherry reporter and CreERT2.DepositorInsertsBFP-SABER
mCherry-t2A-CreERT2
UseZebrafish expressionTagsExpressionMutationPromoterOlactb and hsp70Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCX:γB2-EC5-VenusΔN3C9-P3
Plasmid#209092PurposemVenus-inserted PcdhgB2DepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsPA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:γB2-EC1-G4S-mTQ2ΔC6
Plasmid#209091PurposemTurquoise2-inserted PcdhgB2DepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsPA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:α4-EC4-GA
Plasmid#208514PurposeddGFP-A-inserted Pcdhα4DepositorInsertPcdha4 (Pcdha4 Mouse)
UseTagsHA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
pCX:γB2ΔEC1ΔICD-EB
Plasmid#208522PurposeEBFP2-fused PcdhγB2 lacking an EC1 domain and an ICD domainDepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsEBFP2ExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:γB2ΔEC1ΔICD-mCh
Plasmid#208521PurposemCherry-fused PcdhγB2 lacking an EC1 domain and an ICD domainDepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only