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pCAG-NCad-GA

Plasmid

#191666

Purpose

ddGFP-A-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Use

Tags

HA and ddGFP-A

Expression

Mammalian

Mutation

Promoter

Chicken β-actin promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-NCad-B1

Plasmid

#191667

Purpose

ddFP-B1-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Use

Tags

HA and ddFP-B1

Expression

Mammalian

Mutation

Promoter

Chicken β-actin promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-NCad-B3

Plasmid

#191668

Purpose

ddFP-B3-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Use

Tags

HA and ddFP-B3

Expression

Mammalian

Mutation

Promoter

Chicken β-actin promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-DTR-GFP

Plasmid

#124364

Purpose

conditional expression of a diphtheria toxin receptor (DTR)–GFP fusion protein

Depositor

Eiman Azim, Thomas Jessell

Article

Azim et al Nature. 2014 Apr 17;508(7496):357-63. do

Has Service

AAV2

Insert

DTR-GFP

Use

AAV

Tags

GFP

Expression

Mutation

Promoter

Chicken B-actin

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pR023_PIE-PUM2

Plasmid

#204819

Purpose

Expression PIE-PUM2 fusion protein in mammalian cells

Depositor

Xiaochang Zhang

Article

Ruan et al Nat Commun. 2023 Jun 6;14(1):3275. doi:

Insert

Pumilio 2 (PUM2 Human)

Use

Tags

HA-Flag-rApobec1 and huADAR2dd

Expression

Mammalian

Mutation

Promoter

chicken β-actin promoter

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGs-Foxp1

Plasmid

#45720

Depositor

Jeremy Dasen

Article

Dasen et al Cell. 2008 Jul 25;134(2):304-16. doi: 10

Insert

FoxP1 (Foxp1 Mouse)

Use

Tags

Expression

Mutation

Promoter

CMV / b-actin

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
pBlueScript-humanAPOBEC1

Plasmid

#134606

Purpose

Plasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activity

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

humanAPOBEC1 (APOBEC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Beta actin

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-NCad-V

Plasmid

#191665

Purpose

mVenus-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Use

Tags

HA and mVenus

Expression

Mammalian

Mutation

Promoter

Chicken β-actin promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL-VinculinTS-I997A

Plasmid

#111832

Purpose

Vinculin tension sensor with point mutation affecting ability to bind actin in lentiviral expression vector

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (I997A) (VCL Chicken)

Use

Lentiviral

Tags

Expression

Mutation

changed vinculin isoleucine 997 to alanine

Promoter

CMV

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCaggs-mGold

Plasmid

#157996

Purpose

Expression of mGold in mammalian cells

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

mGold

Use

Tags

Expression

Mammalian

Mutation

mGold is mVenus with L46F;T63S mutations

Promoter

Actin promoter with CMV enhancer

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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tdTomato-Lifeact-7

Plasmid

#54528

Purpose

Localization: Actin, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

Type

Empty backbone

Use

Tags

tdTomato-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pOC1 Halo-Actb KI

Plasmid

#183426

Purpose

CreON knock-in for Halo-beta-actin (amino acid position: before start codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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eMagB-iRFP-Mito

Plasmid

#162250

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagB-iRFP-OMP25

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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ITPKA-mScarlet-I

Plasmid

#98829

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

ITPKA

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLifeAct-miRFP703

Plasmid

#79993

Purpose

F-Actin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Lifeact

Use

Tags

miRFP703

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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LifeAct-LSSmGFP

Plasmid

#186534

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Use

Tags

LifeAct

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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mScarlet-I-UtrCH

Plasmid

#98823

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

UtrCH

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-UtrCH

Plasmid

#98879

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

UtrCH

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

*(see below)

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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eMagA-mCherry-Mito

Plasmid

#162251

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagA-mCherry-OMP25

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEIAV-SIN6.1 CBLacZ-LucWm1A

Plasmid

#44167

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Inserts

LacZ
firefly luciferase

Use

Lentiviral and Luciferase

Tags

Expression

Mutation

Promoter

CB, CMV enhancer/chicken beta-actin promoter

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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