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pconst #1-RFP

Plasmid

#81075

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Jay Keasling, Paul Adams

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

Promoter

Constitutive Promoter - BBa_J23119

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pICH67131

Plasmid

#153223

Purpose

Level 1 position 1 containing the Nos promoter, NPTII (Kan resistance), and the Nos terminator

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Nos promoter, NPTII, Nos terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pME-APEX2-mCherry

Plasmid

#188944

Purpose

Middle entry vector for Gateway cloning containing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry

Depositor

Owen Tamplin

Article

Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

Insert

mito-APEX2_p2A_APEX2-H2B_p2A_mCherry

Use

Middle entry vector for gateway (l1-l2)

Tags

Expression

Mutation

Promoter

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCALNL-eGFP-NT1-6-gix-6-NT1

Plasmid

#81204

Purpose

contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

gix-Neo-gix deletion cassette upstream of EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCBa

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #3-RFP

Plasmid

#81077

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Jay Keasling, Paul Adams

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

Promoter

Constitutive Promoter - BBa_J23101

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #2-RFP

Plasmid

#81076

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Jay Keasling, Paul Adams

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

Promoter

Constitutive Promoter- BBa_J23104

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBER

Plasmid

#62662

Purpose

Donor vector for tdTomato knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Inserts

tdTomato
hygromycin

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBGO

Plasmid

#62671

Purpose

Donor vector for mFTH1 knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

FTH1

Use

Cre/Lox

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pJT309 RapInducible1-ActivatorCloneSite-Citrine

Plasmid

#187321

Purpose

Rapamycin inducible genetic circuit with cloning site for activator domains

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al bioRxiv. (PrePrint)

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBC135

Plasmid

#202262

Purpose

Level 2 for strain-specific barcode DNA tag with bc162 through Tn7 bacterial insertion, Kanamycin selectable marker and tagBFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc162

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC155

Plasmid

#202274

Purpose

Level 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc182

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC113

Plasmid

#202275

Purpose

Level 2 for strain-specific barcode DNA tag with bc140 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc140

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC114

Plasmid

#202276

Purpose

Level 2 for strain-specific barcode DNA tag with bc141 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc141

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBC115

Plasmid

#202277

Purpose

Level 2 for strain-specific barcode DNA tag with bc142 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc142

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC117

Plasmid

#202278

Purpose

Level 2 for strain-specific barcode DNA tag with bc144 through Tn7 bacterial insertion, Tetracyline selectable marker and GFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc144

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC118

Plasmid

#202279

Purpose

Level 2 for strain-specific barcode DNA tag with bc145 through Tn7 bacterial insertion, Tetracyline selectable marker and GFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc145

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBC119

Plasmid

#202280

Purpose

Level 2 for strain-specific barcode DNA tag with bc146 through Tn7 bacterial insertion, Tetracyline selectable marker and GFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc146

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBC108

Plasmid

#202247

Purpose

Level 2 for strain-specific barcode DNA tag with bc135 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc135

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBC160

Plasmid

#202248

Purpose

Level 2 for strain-specific barcode DNA tag with bc187 through Tn7 bacterial insertion, Gentamicin selectable marker and AmCyan1 fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc187

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBC161

Plasmid

#202249

Purpose

Level 2 for strain-specific barcode DNA tag with bc188 through Tn7 bacterial insertion, Gentamicin selectable marker and AmCyan1 fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc188

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge