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Plasmid#176662PurposeExpression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only
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pHS1528
Plasmid#205982PurposeU6-MWEE01000013 Cas5-HNH DR-BpiI-DRDepositorInsertCas5-HNH DR-BpiI-DR
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgRNA scaffold-spacer-human U6
Plasmid#214697Purposeprovide "sgRNA scaffold-spacer-human U6" fragmentDepositorInsertsgRNA scaffold-spacer-human U6
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTetO-hOSK-CASH-1
Plasmid#188977PurposeA CASH-1 GSH targeting, doxycycling inducible humanized Oct4, Sox2, and Klf4 expressing CRISPR/Cas9 donor plasmid.DepositorInsertDoxycycline inducible reprogramming donor cassette
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJB332
Plasmid#115949PurposeORF insert for replicon MoCloDepositorInsertLvl0 TetR-Gly9-DDX6 (contains SapI)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW122
Plasmid#115962PurposeORF insert for replicon MoCloDepositorInsertLvl0 5'-2xTetApt-mVenus-PEST
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILGFPI7
Plasmid#83555PurposeUsed to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.DepositorInsertCUP1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterCUP1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mTagBFP2
Plasmid#175509PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Spike-VLP
Plasmid#173922PurposeExpresses virus-like particles based upon the original SARS-Cov-2DepositorInsertsUseTagsno tags, native proteinExpressionBacterial and MammalianMutationPromoter22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…Available sinceApril 11, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-27:SLC25A17-mEGFP
Plasmid#101784PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human SLC25A17DepositorInsertSLC25A17 Homology Arms with linker-mEGFP (SLC25A17 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRU5-inGFPt
Plasmid#60236PurposeHTLV-1 based transfer vector encoding gfp-turbo reporter gene interrupted by intronDepositorInsertinGFPt
UseRetroviralTagsExpressionMutationPromoterAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2-MG-IRES2-mNeonGreen
Plasmid#175506PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
AA295
Plasmid#215986PurposeFragmid fragment: (N' terminus) SV40 Nuclear Localization SignalDepositorHas ServiceCloning Grade DNAInsertStart; SV40NLS_v1.19
UseFragmentTagsExpressionMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA007
Plasmid#216002PurposeFragmid fragment: (Cas protein) canonical Cas9; NGG PAMDepositorInsertCas9_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA032
Plasmid#216012PurposeFragmid fragment: (Cas protein) deactivated CasDepositorInsertdCas9_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA065
Plasmid#215965PurposeFragmid fragment: (Pol 2 promoter) human EF1a promoterDepositorHas ServiceCloning Grade DNAInsertEF1a_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA-XMAS-1x
Plasmid#164413PurposeDual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry
Plasmid#177182Purposeplasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry markerDepositorInsertpegRNA targeting the PEAR-GFP plasmid
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMTMVi-M
Plasmid#118757PurposeIntermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plantDepositorInsertTobacco mosaic virus cDNA fragment with a cloning polylinker
UseIntermediate to build tobacco mosaic virus recomb…TagsExpressionMutationPromoterAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-41: ATP2A2-mEGFP
Plasmid#114405PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human ATP2A2DepositorInsertATP2A2 Homology Arms with mEGFP-linker (ATP2A2 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceSept. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDAS12342_PEAR-GFP_2in1
Plasmid#177184PurposePEAR-GFP plasmid with a pegRNA that targets its own plasmidDepositorInsertsEGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid
UseCRISPRTagsExpressionMammalianMutationdisrupted 5' splice sitePromoterCMV and U6Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD315 (mTurquoise2^SEC^2xHA)
Plasmid#73343PurposemTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology armsDepositorInsertmTurquoise2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags2xHA and C. elegans codon-optimized mTurquoise2ExpressionWormMutationPromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZCS38 (Prsp-27::NeoR::unc-54 3’ UTR)
Plasmid#193049PurposeEncodes G-418 resistanceDepositorInsertprsp-27::NeoR::unc-54 3’ UTR
UseTagsExpressionWormMutationPromoterprsp-27Available sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJW31
Plasmid#136423PurposeContains genes of Type I-C CRISPR-Cas system for inducible expression and chromosomal integration at attTn7DepositorInsertsCas5
Cas8
Cas7
Cas3
Cas3
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
AA015
Plasmid#216058PurposeFragmid fragment: (2A Selection) Puromycin resistance, T2A, EGFPDepositorInsertP2A_v1.1; PuroR_v1.1; T2A_v1.2; EGFP_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA011
Plasmid#215973PurposeFragmid fragment: (N' terminus) for C>T base editingDepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1; rAPOBEC1_v1.1; Linker_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA041
Plasmid#215963PurposeFragmid fragment: (Pol 2 promoter) human core (short) EF1a promoterDepositorHas ServiceCloning Grade DNAInsertEFS_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA406
Plasmid#216070PurposeFragmid fragment: (2A Selection) Violet-excited GFP, T2A, blasticidin resistanceDepositorInsertP2A_v1.1; VexGFP_v1.1; T2A_v1.1; BlastR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA013
Plasmid#215975PurposeFragmid fragment: (N' terminus) for A>G base editingDepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1; ABE8e_v1.1; Linker_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFP91
Plasmid#83548PurposeUsed to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.DepositorInsertPGK1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterPGK1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfp(ASV)C
Plasmid#111615PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILGFP92
Plasmid#83549PurposeUsed to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.DepositorInsertTDH3 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTDH3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPotef-cdc3CB16-NatR
Plasmid#86465PurposeExpresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.DepositorInsertCdc3
UseUstilago maydis expressionTagsmCherryExpressionBacterialMutationPromoterPotefAvailable sinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS2337_Tier3(SB)-TagBFP2_Puro
Plasmid#169635PurposeTier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mTagBFP2 and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRU5-inmCherry
Plasmid#60238PurposeHTLV-1 based transfer vector encoding mcherry reporter gene interrupted by intronDepositorInsertinmCherry
UseRetroviralTagsExpressionMutationPromoterAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT9679-sgRNA-BFP
Plasmid#162988PurposesgRNA cloning plasmidDepositorInsertexchangeable cassette
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Consensus
Plasmid#176664PurposeExpression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFPB5A
Plasmid#83556PurposeUsed to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.DepositorInsertGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL1Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-22:CETN2-mTagRFP-T
Plasmid#101781PurposeHomology arms and mTagRFPT-linker sequence for N-terminus tagging of human CETN2DepositorInsertCETN2 Homology Arms with mTagRFPT-linker (CETN2 Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMTMVi-N
Plasmid#118756PurposeIntermediate plasmid to build tobacco mosaic virus recombinant clones that do not move systemically throughout the plantDepositorInsertTobacco mosaic virus cDNA fragment with a cloning polylinker
UseIntermediate to build tobacco mosaic virus recomb…TagsExpressionMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Agam_695
Plasmid#176654PurposeExpression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP97
Plasmid#83551PurposeUsed to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.DepositorInsertCYC1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterCYC1Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPAP_BGL1
Plasmid#183735PurposeExpresses Aspergillus aculeatus β-glucosidase (BGL1)DepositorInsertβ-glucosidase (AaBGL1)
UseTagsExpressionYeastMutationPromoterAOX1 promoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-83:EZH2-mEGFP
Plasmid#164499PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human EZH2DepositorInsertEZH2 Homology Arms with mEGFP-linker (EZH2 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceFeb. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDGE334
Plasmid#153243PurposeM2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aaeg_774
Plasmid#176659PurposeExpression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)
Plasmid#164040PurposeExpression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2DepositorInsertsgTS5-sgTS6-sgTS7
UseCRISPRTagsExpressionMutationPromotermouse U6Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
BC1450-H2B 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)
Plasmid#164041PurposeCRISPR donor plasmid to insert mCherry-CRISPR-TagV2 into the C-terminus of human H2B geneDepositorInsertmCherry-CRISPR-TagV2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFPH7
Plasmid#83554PurposeUsed to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.DepositorInsertADH2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterADH2Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE331
Plasmid#153240PurposeM1E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only