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pCMV-BLZinCh-P50

Plasmid

#188058

Purpose

Expression of BLZinCh-P50, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P50

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-CrPV-Rluc-6xbsMS2-PolyA-Malat_AC

Plasmid

#148446

Purpose

Mammalian Expression of 6xbsMS2-PolyA-Malat

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-PolyA-Malat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HCV-RLuc-6xbsMS2-polyA-Malat_AC

Plasmid

#148448

Purpose

Mammalian Expression of 6xbsMS2-polyA-Malat

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-polyA-Malat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P20

Plasmid

#188055

Purpose

Expression of BLZinCh-P20, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P20

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-BLZinCh-P30

Plasmid

#188056

Purpose

Expression of BLZinCh-P30, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P30

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S46C

Plasmid

#190008

Purpose

Expression of human TTR in E coli

Depositor

Peter Wright

Article

Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, S46C

Promoter

T7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S E63C

Plasmid

#190009

Purpose

Expression of human TTR in E coli

Depositor

Peter Wright

Article

Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, E63C

Promoter

T7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-mGreenLantern332

Plasmid

#188483

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern sgRNA

Use

CRISPR

Tags

PB_rtTA_BsmBI

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-CMV152

Plasmid

#188485

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
rtTA-sg-tdTomato166/892

Plasmid

#188488

Purpose

gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 166 & 892.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

tdTomato sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
rtTA-sg-tdTomato90/816

Plasmid

#188487

Purpose

gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 90 & 816.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

tdTomato sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
rtTA-sg-CMV162

Plasmid

#188486

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
rtTA-sg-CMV51

Plasmid

#188484

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
rtTA-sg-mGreenLantern/EGFP

Plasmid

#188482

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern/EGFP sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV‐ABEmaxNGX‐NGA

Plasmid

#187059

Purpose

ABEmax-SpCas9-NGX‐NGA

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

ABEmaxNGX‐NGA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV‐ABEmaxNGX‐NGC

Plasmid

#187060

Purpose

ABEmax-SpCas9-NGX-NGC

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

ABEmaxNGX‐NGC

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV‐ABEmaxNGX

Plasmid

#187058

Purpose

ABEmax-SpCas9-NGX

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

ABEmaxNGX

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV‐ABEmaxNGX‐loop

Plasmid

#187061

Purpose

ABEmax-SpCas9-NGX-loop

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

ABEmaxNGX‐loop

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_ABE‐NGC

Plasmid

#187056

Purpose

ABEmax-SpCas9-NGC

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

ABE‐NGC

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET30-T7-lacO-His-sfGFP

Plasmid

#180754

Purpose

pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Use

Tags

strep tag

Expression

Bacterial

Mutation

G140C- Please see depositor comments

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP.N3-myc-SLC35A2

Plasmid

#186285

Purpose

transient expression of N-terminal myc tagged SLC35A2 isoform c/UGT1 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al Glycobiology. 2019 Jun 1;29(6):490-503.

Insert

SLC35A2 (SLC35A2 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAW2-Smed-Slit-1

Plasmid

#182264

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Slit-1

Depositor

Daniel Lobo

Article

Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do

Insert

Smed-Slit-1

Use

Gibson cloning vector for synthesis of single and…

Tags

Expression

Mutation

Promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK23 pCAS-Tyr-[gRNA: 6=ARS416] (SplitHygR, AmpR)

Plasmid

#179007

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK22 pCAS-Tyr-[gRNA: 5=ARS308] (SplitHygR, AmpR)

Plasmid

#179006

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK19 pCAS-Tyr-[gRNA: 2=FF21] (SplitHygR, AmpR)

Plasmid

#179003

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

Plasmid

#179002

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK17 pCAS-Tyr-[gRNA: 7=HIS3] (SplitKanR, AmpR)

Plasmid

#179001

Purpose

Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK16 pCAS-Tyr-[gRNA: 6=ARS416] (SplitKanR, AmpR)

Plasmid

#179000

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK15 pCAS-Tyr-[gRNA: 5=ARS308] (SplitKanR, AmpR)

Plasmid

#178999

Purpose

Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-sg-CMV152

Plasmid

#179916

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-sg-CMV51

Plasmid

#179915

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-sg-CMV162

Plasmid

#179917

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-sg-mGreenLantern332

Plasmid

#179914

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PX552-rScn2a-3xV5-Syn-miRFP670

Plasmid

#182566

Purpose

For rat Nav1.2 knockin with 3xV5 at the extracellular loop

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

rat Scn2a gRNA and 3xV5 donor DNA
miRFP670

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Syn and U6

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
piggybac-Syn-mScn8a_ICL1-sfGFP-P2A-mCherry

Plasmid

#182560

Purpose

Expresses Nav1.6 ICL1 tagged with sfGFP-P2A-mCherry

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a ICL1
sfGFP-P2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Syn

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG.mCherry-INBox-eDHFR

Plasmid

#176202

Purpose

Single INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

mCherry-INBox-eDHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG.MCAK-mCherry-eDHFR

Plasmid

#176204

Purpose

MCAK (179-583) recruitment responsible for targeted microtubule depolymerization using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

MCAK-mCherry-eDHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-EGFP-PML peptide

Plasmid

#175247

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

PML (PML Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-RanGAP*

Plasmid

#175237

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the Km

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-FKBP-E2

Plasmid

#175243

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH858

Plasmid

#172176

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH859

Plasmid

#172177

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH580

Plasmid

#172164

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 3f2v2+B); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K, E178K, G188R, E251D, G332…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH581

Plasmid

#172165

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 3f2+C); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH750

Plasmid

#172170

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.3f2, deleted sumo

Use

Tags

Expression

Bacterial

Mutation

Evolved PylBCD operon; SUMO tag deleted from PylB…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH751

Plasmid

#172171

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.JM10.1, deleted sumo

Use

Tags

Expression

Bacterial

Mutation

Evolved PylBCD operon; SUMO tag deleted from PylB…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH768v2

Plasmid

#172173

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.3f2]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH769v2

Plasmid

#172174

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH61

Plasmid

#172143

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] pylS; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

gIII(P29*); [strong promoter eG, strong RBS SD8] …

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Bax gRNA#1

Plasmid

#171827

Purpose

Cas9-mediated knockout of Bax in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bax (Bax Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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