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Plasmid#215100PurposeExpresses FokI(inactive)-mCherry-LacI-NLS in mammalian cells.DepositorInsertFokI(inactive)-mCherry-LacI-NLS
UseTagsExpressionMammalianMutationFokI(inactive) mutant D69A.PromoterCMVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
CDKN1A (p21) targeting gRNA
Plasmid#215319PurposeExpresses gRNA targeting CDKN1A (p21) and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.DepositorInserthSpCas9
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterCbhAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AcGFP-MSH2
Plasmid#215121PurposeExpresses AcGFP-MSH2 in mammalian cells from a retroviral vector.DepositorInsertMSH2 (MSH2 Human)
UseRetroviralTagsAcGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJW1583
Plasmid#121054PurposeGFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertGFP^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, C. elegans codon optimized GFP, and auxin…ExpressionWormMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AR-LBD
Plasmid#89111Purposemammalian expression of human androgen receptor ligand binding domain amino acid residues 624-919 with N-terminal double HA tagDepositorInserthAR ligand binding domain (AR Human)
UseTags2xHAExpressionMammalianMutationcontains Ligand Binding Domain (624-919)PromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJW1584
Plasmid#121055PurposeYPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertYPET^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, C. elegans codon optimized YPET, and auxi…ExpressionWormMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-CDK2
Plasmid#172613PurposeExpresses HA-tagged CDK2 in mammalian cellsDepositorInsertCDK2 (CDK2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCC324
Plasmid#110165PurposeminiMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)DepositorInsertERK-KTR-mClover-T2A-mCherry-H2B
UseMinimos transposonTagsmCherry and mCloverExpressionWormMutationR61K change made in ERK-KTR CDSPromoterlin-31 promoter/enhancerAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CCND1
Plasmid#172632PurposeExpresses untagged cyclin D1 in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
NTHL1-mCherry
Plasmid#215145PurposeExpresses NTHL1-mCherry in mammalian cells from a retroviral vector.DepositorInsertNTHL1 (NTHL1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only